With advances in sequencing and instrument technology, bioinformatics analysis is being
applied to batches of massive cells at single-cell resolution. High-throughput single-cell …

Droplet microfluidics has revolutionized the study of single cells. The ability to
compartmentalize cells within picoliter droplets in microfluidic devices has opened up a wide …

Genome instability and aberrant alterations of transcriptional programs both play important
roles in cancer. Single-cell RNA sequencing (scRNA-seq) has the potential to investigate …

Single-cell RNA sequencing has enabled the characterization of highly specific cell types in
many tissues, as well as both primary and stem cell-derived cell lines. An important facet of …

Purpose: The tumor microenvironment (TME) consists of a heterogenous cellular milieu that
can influence cancer cell behavior. Its characteristics have an impact on treatments such as …

The clonal evolution model of cancer was developed in the 1950s–1970s and became
central to cancer biology in the twenty-first century, largely through studies of cancer …

Single-cell sequencing (SCS) has impacted many areas of cancer research and improved
our understanding of intratumor heterogeneity, the tumor microenvironment, metastasis, and …

Gastric cancer is a major source of global cancer mortality with limited treatment options and
poor patient survival. As our molecular understanding of gastric cancer improves, we are …

Single-cell barcoding technologies enable genome sequencing of thousands of individual
cells in parallel, but with extremely low sequencing coverage (< 0.05×) per cell. While the …

Ductal carcinoma in situ (DCIS) is a common precursor of invasive breast cancer. Our
understanding of its genomic progression to recurrent disease remains poor, partly due to …