The eukaryotic 5′ untranslated region (UTR) is critical for ribosome recruitment to the
messenger RNA (mRNA) and start codon choice and plays a major role in the control of …

Traditional annotation of protein-encoding genes relied on assumptions, such as one open
reading frame (ORF) encodes one protein and minimal lengths for translated proteins. With …

In addition to the main, protein-coding, open reading frame (mORF), many eukaryotic
mRNAs contain upstream ORFs (uORFs) initiated at AUG or near-cognate codons residing …

Short open reading frames (sORFs) are a common feature of all genomes, but their coding
potential has mostly been disregarded, partly because of the difficulty in determining …

Translational control in eukaryotic cells is critical for gene regulation during nutrient
deprivation and stress, development and differentiation, nervous system function, aging, and …

Ever since the beginning of biochemical analysis, yeast has been a pioneering model for
studying the regulation of eukaryotic metabolism. During the last three decades, the …

Although most mRNA molecules derived from protein-coding genes are destined to be
translated into functional polypeptides, some are eliminated by cellular quality control …

Nonsense-mediated mRNA decay (NMD) is a eukaryotic surveillance mechanism that
monitors cytoplasmic mRNA translation and targets mRNAs undergoing premature …

All RNA species in yeast cells are subject to turnover. Work over the past 20 years has
defined degradation mechanisms for messenger RNAs, transfer RNAs, ribosomal RNAs …

Deep sequencing of ribosome footprints (ribosome profiling) maps and quantifies mRNA
translation. Because ribosomes decode mRNA every 3 nt, the periodic property of ribosome …