Dependence of avidity on linker length for a bivalent ligand–bivalent receptor model system

ET Mack, PW Snyder, R Perez-Castillejos… - Journal of the …, 2012 - ACS Publications
This paper describes a synthetic dimer of carbonic anhydrase, and a series of bivalent
sulfonamide ligands with different lengths (25 to 69 Å between the ends of the fully extended …

Reversible blocking of antibodies using bivalent peptide–DNA conjugates allows protease-activatable targeting

BMG Janssen, EHM Lempens, LLC Olijve, IK Voets… - Chemical …, 2013 - pubs.rsc.org
Antibody-based molecular recognition plays a dominant role in the life sciences ranging
from applications in diagnostics and molecular imaging to targeted drug delivery and …

Biophysical considerations for development of antibody-based therapeutics

AW Drake, YN Abdiche, GA Papalia - Development of Antibody-Based …, 2018 - Springer
Abstract Development of therapeutic monoclonal antibodies requires rigorous
measurements of the kinetic and thermodynamic binding properties of antibody–antigen …

A general model for predicting the binding affinity of reversibly and irreversibly dimerized ligands

KW Foreman - PLoS One, 2017 - journals.plos.org
Empirical data has shown that bivalent inhibitors can bind a given target protein significantly
better than their monomeric counterparts. However, predicting the corresponding theoretical …

Divalent ligand-monovalent molecule binding

M Janssen, H Stenmark, A Carlson - Soft Matter, 2021 - pubs.rsc.org
Simultaneous binding of a divalent ligand to two identical monovalent molecules is a
widespread phenomenon in biology and chemistry. Here, we describe how two such …

Quantitative analysis of affinity enhancement by noncovalently oligomeric ligands

GMK Poon - Analytical biochemistry, 2013 - Elsevier
Designed ligands that self-assemble noncovalently via an independent oligomerization
domain have demonstrated enhancement in affinity for a variety of chemical and biological …

Thermodynamic analysis to assist in the design of recombinant antibodies

ET Mack, PJ Bracher… - Critical Reviews™ in …, 2012 - dl.begellhouse.com
This critical review examines the thermodynamics of binding of bivalent antibodies (IgG and
IgE) to soluble ligands with two or more binding moieties joined covalently (multivalent …

Considerations for construct and affinity design goals

MA Tabrizi, SL Klakamp - Development of Antibody-Based Therapeutics …, 2018 - Springer
Application of an integrated approach for inclusion of pharmacology principles in drug
development is a foundational step for effective modality design and selection of antibody …

Analysis of biochemical equilibria relevant to the immune response: finding the dissociation constants

LJ Cummings, R Perez-Castillejos, ET Mack - Bulletin of mathematical …, 2012 - Springer
This paper analyzes the biochemical equilibria between bivalent receptors, homo-
bifunctional ligands, monovalent inhibitors, and their complexes. Such reaction schemes …

[PDF][PDF] Dependence of Avidity on Linker Length for a Bivalent Ligand–Bivalent Receptor Model System

DTM Bilgiçer, MJ Butte, M George - core.ac.uk
This paper describes a synthetic dimer of carbonic anhydrase, and a series of bivalent
sulfonamide ligands with different lengths (25 to 69 Å between the ends of the fully extended …