Current development in genetic engineering strategies of Bacillus species

H Dong, D Zhang - Microbial cell factories, 2014 - Springer
The complete sequencing and annotation of the genomes of industrially-important Bacillus
species has enhanced our understanding of their properties, and allowed advances in …

Essential genes as antimicrobial targets and cornerstones of synthetic biology

M Juhas, L Eberl, GM Church - Trends in biotechnology, 2012 - cell.com
Essential genes are absolutely required for the survival of any living entity. Investigation of
essential genes is therefore expected to advance tremendously our understanding of the …

MreB filaments align along greatest principal membrane curvature to orient cell wall synthesis

S Hussain, CN Wivagg, P Szwedziak, F Wong… - Elife, 2018 - elifesciences.org
MreB is essential for rod shape in many bacteria. Membrane-associated MreB filaments
move around the rod circumference, helping to insert cell wall in the radial direction to …

A host-vector toolbox for improved secretory protein overproduction in Bacillus subtilis

A Krüger, N Welsch, A Dürwald, H Brundiek… - Applied microbiology …, 2022 - Springer
Target proteins in biotechnological applications are highly diverse. Therefore, versatile
flexible expression systems for their functional overproduction are required. In order to find …

A dynamic pathway analysis approach reveals a limiting futile cycle in N-acetylglucosamine overproducing Bacillus subtilis

Y Liu, H Link, L Liu, G Du, J Chen, U Sauer - Nature communications, 2016 - nature.com
Recent advances in genome engineering have further widened the gap between our ability
to implement essentially any genetic change and understanding the impact of these …

Rewiring the Glucose Transportation and Central Metabolic Pathways for Overproduction of N‐Acetylglucosamine in Bacillus subtilis

Y Gu, J Deng, Y Liu, J Li, H Shin, G Du… - Biotechnology …, 2017 - Wiley Online Library
N‐acetylglucosamine (GlcNAc) is an important amino sugar extensively used in the
healthcare field. In a previous study, the recombinant Bacillus subtilis strain BSGN6‐PxylA …

Construction of a novel, stable, food-grade expression system by engineering the endogenous toxin-antitoxin system in Bacillus subtilis

S Yang, Z Kang, W Cao, G Du, J Chen - Journal of biotechnology, 2016 - Elsevier
Bacillus subtilis as an important workhorse that has been widely used to produce enzymes
and metabolites. To broaden its applications, especially in the food and feed industry, we …

A simple method of markerless gene deletion in Staphylococcus aureus

F Kato, M Sugai - Journal of microbiological methods, 2011 - Elsevier
Staphylococcus aureus is a Gram-positive pathogen that causes opportunistic infections and
a wide variety of diseases. Methicillin-resistant S. aureus (MRSA) is frequently isolated as …

Establishment of a Markerless Mutation Delivery System in Bacillus subtilis Stimulated by a Double-Strand Break in the Chromosome

T Shi, G Wang, Z Wang, J Fu, T Chen, X Zhao - PloS one, 2013 - journals.plos.org
Bacillus subtilis has been a model for gram-positive bacteria and it has long been exploited
for industrial and biotechnological applications. However, the availability of facile genetic …

Engineering of Yersinia Phytases to Improve Pepsin and Trypsin Resistance and Thermostability and Application Potential in the Food and Feed Industry

C Niu, P Yang, H Luo, H Huang… - Journal of agricultural …, 2017 - ACS Publications
Susceptibility to proteases usually limits the application of phytase. We sought to improve
the pepsin and trypsin resistance of YeAPPA from Yersinia enterocolitica and YkAPPA from …