The maintenance of genome integrity involves multiple independent DNA damage
avoidance and repair mechanisms. However, the origin and pathways of the focal …

Here, we survey the diverse functions of DNA polymerase ζ (pol ζ) in eukaryotes. In
mammalian cells, REV3L (3130 residues) is the largest catalytic subunit of the DNA …

Synthetic lethality strategies for cancer therapy exploit cancer-specific genetic defects to
identify targets that are uniquely essential to the survival of tumor cells. Here we show …

Despite next-generation sequencing, which now allows for the accurate detection of
segmental aneuploidies from in vitro fertilization embryo biopsies, the origin and …

Displacement loops (D-loops) are pivotal intermediates of homologous recombination (HR),
a universal DNA double strand break (DSB) repair pathway. We developed a versatile assay …

To investigate the fidelity of canonical non-homologous end joining (C-NHEJ), we
developed an assay to detect EJ between distal ends of two Cas9-induced chromosomal …

DNA damage provokes mutations and cancer and results from external carcinogens or
endogenous cellular processes. However, the intrinsic instigators of endogenous DNA …

Maintenance of genomic integrity involves cellular processes tailored to specific types of
DNA damage. Monogenic disorders in DNA-repair pathways lead to a spectrum of clinical …

STUDY QUESTION What is the true incidence of chromosomal mosaicism in embryos
analyzed by preimplantation genetic testing (PGT) SUMMARY ANSWER The true incidence …

Genome rearrangements result in mutations that underlie many human diseases, and
ongoing genome instability likely contributes to the development of many cancers. The tools …