Advances in industrial biotechnology using CRISPR-Cas systems
PD Donohoue, R Barrangou, AP May - Trends in biotechnology, 2018 - cell.com
The term 'clustered regularly interspaced short palindromic repeats'(CRISPR) has recently
become synonymous with the genome-editing revolution. The RNA-guided endonuclease …
become synonymous with the genome-editing revolution. The RNA-guided endonuclease …
Applications of CRISPR/Cas system to bacterial metabolic engineering
The clustered regularly interspaced short palindromic repeats/CRISPR-associated
(CRISPR/Cas) adaptive immune system has been extensively used for gene editing …
(CRISPR/Cas) adaptive immune system has been extensively used for gene editing …
A thermostable Cas9 with increased lifetime in human plasma
LB Harrington, D Paez-Espino, BT Staahl… - Nature …, 2017 - nature.com
CRISPR-Cas9 is a powerful technology that has enabled genome editing in a wide range of
species. However, the currently developed Cas9 homologs all originate from mesophilic …
species. However, the currently developed Cas9 homologs all originate from mesophilic …
[HTML][HTML] CRISPR-Cas9/Cas12a biotechnology and application in bacteria
R Yao, D Liu, X Jia, Y Zheng, W Liu, Y Xiao - Synthetic and systems …, 2018 - Elsevier
CRISPR-Cas technologies have greatly reshaped the biology field. In this review, we
discuss the CRISPR-Cas with a particular focus on the associated technologies and …
discuss the CRISPR-Cas with a particular focus on the associated technologies and …
Genetic engineering of extremely acidophilic Acidithiobacillus species for biomining: Progress and perspectives
With global demands for mineral resources increasing and ore grades decreasing,
microorganisms have been increasingly deployed in biomining applications to recover …
microorganisms have been increasingly deployed in biomining applications to recover …
Characterizing a thermostable Cas9 for bacterial genome editing and silencing
CRISPR-Cas9-based genome engineering tools have revolutionized fundamental research
and biotechnological exploitation of both eukaryotes and prokaryotes. However, the …
and biotechnological exploitation of both eukaryotes and prokaryotes. However, the …
Recent Developments of the Synthetic Biology Toolkit for Clostridium
The Clostridium genus is a large, diverse group consisting of Gram-positive, spore-forming,
obligate anaerobic firmicutes. Among this group are historically notorious pathogens as well …
obligate anaerobic firmicutes. Among this group are historically notorious pathogens as well …
Barriers to genome editing with CRISPR in bacteria
Genome editing is essential for probing genotype–phenotype relationships and for
enhancing chemical production and phenotypic robustness in industrial bacteria. Currently …
enhancing chemical production and phenotypic robustness in industrial bacteria. Currently …
CRISPR-Cas9 system: a genome-editing tool with endless possibilities
The discovery of CRISPR: Cas9 and its application as a powerful gene-editing tool has
transformed the world of basic and applied science, especially the molecular biology dome …
transformed the world of basic and applied science, especially the molecular biology dome …
Bacterial genome reductions: Tools, applications, and challenges
N LeBlanc, TC Charles - Frontiers in Genome Editing, 2022 - frontiersin.org
Bacterial cells are widely used to produce value-added products due to their versatility, ease
of manipulation, and the abundance of genome engineering tools. However, the efficiency of …
of manipulation, and the abundance of genome engineering tools. However, the efficiency of …