Fluorescence lifetime imaging microscopy

B Torrado, B Pannunzio, L Malacrida… - Nature Reviews …, 2024 - nature.com
Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique offering profound
insights into a broad spectrum of biological processes such as metabolic imaging, protein …

Fluorescence lifetime imaging with a megapixel SPAD camera and neural network lifetime estimation

V Zickus, ML Wu, K Morimoto, V Kapitany, A Fatima… - Scientific Reports, 2020 - nature.com
Fluorescence lifetime imaging microscopy (FLIM) is a key technology that provides direct
insight into cell metabolism, cell dynamics and protein activity. However, determining the …

High-speed imaging of transient metabolic dynamics using two-photon fluorescence lifetime imaging microscopy

AJ Bower, J Li, EJ Chaney, M Marjanovic… - Optica, 2018 - opg.optica.org
Two-photon fluorescence lifetime imaging microscopy (2P-FLIM) of autofluorescent
metabolic coenzymes has been widely used to investigate energetic perturbations in living …

Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates

S Karpf, CT Riche, D Di Carlo, A Goel… - Nature …, 2020 - nature.com
Abstract Two-Photon Microscopy has become an invaluable tool for biological and medical
research, providing high sensitivity, molecular specificity, inherent three-dimensional sub …

High-resolution retinal swept source optical coherence tomography with an ultra-wideband Fourier-domain mode-locked laser at MHz A-scan rates

JP Kolb, T Pfeiffer, M Eibl, H Hakert… - Biomedical optics …, 2017 - opg.optica.org
We present a new 1060 nm Fourier domain mode locked laser (FDML laser) with a record
143 nm sweep bandwidth at 2∙ 417 kHz= 834 kHz and 120 nm at 1.67 MHz, respectively. We …

[HTML][HTML] Modeling cardiac complexity: Advancements in myocardial models and analytical techniques for physiological investigation and therapeutic development in …

NI Callaghan, S Hadipour-Lakmehsari, SH Lee… - APL …, 2019 - pubs.aip.org
Cardiomyopathies, heart failure, and arrhythmias or conduction blockages impact millions of
patients worldwide and are associated with marked increases in sudden cardiac death …

Single-photon peak event detection (SPEED): a computational method for fast photon counting in fluorescence lifetime imaging microscopy

JE Sorrells, RR Iyer, L Yang, EJ Chaney… - Optics …, 2021 - opg.optica.org
Fluorescence lifetime imaging microscopy (FLIM) characterizes samples by examining the
temporal properties of fluorescence emission, providing useful contrast within samples …

Development of a high-speed line-scanning fluorescence lifetime imaging microscope for biological imaging

H Mai, A Jarman, AT Erdogan, C Treacy, N Finlayson… - Optics letters, 2023 - opg.optica.org
We report the development of a novel line-scanning microscope capable of acquiring high-
speed time-correlated single-photon counting (TCSPC)-based fluorescence lifetime imaging …

Widefield fluorescence lifetime imaging of protoporphyrin IX for fluorescence‐guided neurosurgery: An ex vivo feasibility study

MT Erkkilä, B Bauer, N Hecker‐Denschlag… - Journal of …, 2019 - Wiley Online Library
Achieving a maximal safe extent of resection during brain tumor surgery is the goal for
improved patient prognosis. Fluorescence‐guided neurosurgery using 5‐aminolevulinic …

Tracking metabolic dynamics of apoptosis with high-speed two-photon fluorescence lifetime imaging microscopy

AJ Bower, JE Sorrells, J Li, M Marjanovic… - Biomedical Optics …, 2019 - opg.optica.org
Programmed cell death, or apoptosis, is an essential process in development and
homeostasis, and disruptions in associated pathways are responsible for a wide variety of …