Precise spatio–temporal control of gene activity is essential for organismal development,
growth, and survival in a changing environment. Decisive steps in gene regulation involve …

Noncoding RNAs (ncRNAs) direct a remarkable number of diverse functions in development
and disease through their regulation of transcription, RNA processing and translation …

In higher eukaryotes, fine-tuned activation of protein-coding genes and many non-coding
RNAs pivots around the regulated activity of RNA polymerase II (Pol II). The Integrator …

The dynamic regulation of transcription elongation by RNA polymerase II (Pol II) is an
integral part of the implementation of gene expression programmes during development. In …

Recent advances in sequencing techniques that measure nascent transcripts and that
reveal the positioning of RNA polymerase II (Pol II) have shown that the pausing of Pol II in …

We provide a protocol for precision nuclear run-on sequencing (PRO-seq) and its variant,
PRO-cap, which map the location of active RNA polymerases (PRO-seq) or transcription …

Cellular development, morphology and function are governed by precise patterns of gene
expression. These are established by the coordinated action of genomic regulatory …

The programmes that direct an organism's development and maintenance are encoded in its
genome. Decoding of this information begins with regulated transcription of genomic DNA …

Regulation by gene-distal enhancers is critical for cell type-specific and condition-specific
patterns of gene expression. Thus, to understand the basis of gene activity in a given cell …

Genomic enhancers are important regulators of gene expression, but their identification is a
challenge, and methods depend on indirect measures of activity. We developed a method …