Enzyme-linked immunosorbent assay for the quantitative/qualitative analysis of plant secondary metabolites

S Sakamoto, W Putalun, S Vimolmangkang… - Journal of natural …, 2018 - Springer
Immunoassays are antibody-based analytical methods for quantitative/qualitative analysis.
Since the principle of immunoassays is based on specific antigen–antibody reaction, the …

Evolution of Escherichia coli Expression System in Producing Antibody Recombinant Fragments

A Sandomenico, JP Sivaccumar, M Ruvo - International Journal of …, 2020 - mdpi.com
Antibodies and antibody-derived molecules are continuously developed as both therapeutic
agents and key reagents for advanced diagnostic investigations. Their application in these …

Single-chain fragment variable: recent progress in cancer diagnosis and therapy

P Muñoz-López, RM Ribas-Aparicio, EI Becerra-Báez… - Cancers, 2022 - mdpi.com
Simple Summary Recombinant antibody fragments have shown remarkable potential as
diagnostic and therapeutic tools in the fight against cancer. The single-chain fragment …

Broad dengue neutralization in mosquitoes expressing an engineered antibody

A Buchman, S Gamez, M Li, I Antoshechkin… - PLoS …, 2020 - journals.plos.org
With dengue virus (DENV) becoming endemic in tropical and subtropical regions worldwide,
there is a pressing global demand for effective strategies to control the mosquitoes that …

Antibody variable region engineering for improving cancer immunotherapy

H Lou, X Cao - Cancer Communications, 2022 - Wiley Online Library
The efficacy and specificity of conventional monoclonal antibody (mAb) drugs in the clinic
require further improvement. Currently, the development and application of novel antibody …

Development of a POCT type insulin sensor employing anti-insulin single chain variable fragment based on faradaic electrochemical impedance spectroscopy under …

M Khanwalker, R Fujita, J Lee, E Wilson, K Ito… - Biosensors and …, 2022 - Elsevier
To improve glycemic control managed through insulin administration, recent studies have
focused on developing hand-held point-of-care testing (POCT) electrochemical biosensors …

Antibodies as snakebite antivenoms: past and future

W Dias da Silva, SA De Andrade, ÂAA Megale… - Toxins, 2022 - mdpi.com
Snakebite envenomation is considered a neglected tropical disease, affecting tens of
thousands of people each year. The recommended treatment is the use of antivenom, which …

Human VP8* mAbs neutralize rotavirus selectively in human intestinal epithelial cells

N Feng, L Hu, S Ding, M Sanyal, B Zhao… - The Journal of …, 2019 - Am Soc Clin Investig
We previously generated 32 rotavirus-specific (RV-specific) recombinant monoclonal
antibodies (mAbs) derived from B cells isolated from human intestinal resections. Twenty …

Effect of non-repetitive linker on in vitro and in vivo properties of an anti-VEGF scFv

M Arslan, M Karadag, E Onal, E Gelinci… - Scientific Reports, 2022 - nature.com
Single chain antibody fragments (scFvs) are favored in diagnostic and therapeutic fields
thanks to their small size and the availability of various engineering approaches. Linker …

The Potential of Single‐Chain Variable Fragment Antibody: Role in Future Therapeutic and Diagnostic Biologics

G Gezehagn Kussia… - Journal of Immunology …, 2024 - Wiley Online Library
The advancement of genetic engineering has revolutionized the field of immunology by
allowing the utilization of intrinsic antibody structures. One of the biologics that are being …