Biotherapeutics and their biosimilar versions have been flourishing in the biopharmaceutical
market for several years. Structural and functional characterization is needed to achieve …

Aggregation of protein-based therapeutics can occur during development, production, or
storage and can lead to loss of efficacy and potential toxicity. Native mass spectrometry of a …

Native electrospray ionization mass spectrometry (ESI-MS) has emerged as a potent tool for
examining the native-like structures of macromolecular complexes. Despite its utility, the …

Broader adoption of native mass spectrometry (MS) and ion mobility‐mass spectrometry (IM‐
MS) has propelled the development of several techniques which take advantage of the …

Fc-fusion proteins are an emerging class of protein therapeutics that combine the properties
of biological ligands with the unique properties of the fragment crystallizable (Fc) domain of …

Capillary vibrating sharp-edge spray ionization (cVSSI) has been used to control the droplet
charging of nebulized microdroplets and monitor effects on protein ion conformation makeup …

One of the most important attributes of anti‐amyloid antibodies is their selective binding to
oligomeric and amyloid aggregates. However, current methods of examining the binding …

Endogenous antibodies, or immunoglobulins (Igs), abundantly present in body fluids,
represent some of the most challenging samples to analyze, largely due to the immense …

Biotherapeutic's higher order structure (HOS) is a critical determinant of its functional
properties and conformational relevance. Here, we evaluated two covalent labeling …

Ion mobility spectrometry coupled to mass spectrometry (IMS/MS) is a widely used tool for
biomolecular separations and structural elucidation. The application of IMS/MS has resulted …