High-resolution native mass spectrometry
Native mass spectrometry (MS) involves the analysis and characterization of
macromolecules, predominantly intact proteins and protein complexes, whereby as much as …
macromolecules, predominantly intact proteins and protein complexes, whereby as much as …
Studying protein structure and function by native separation–mass spectrometry
G van Schaick, R Haselberg, GW Somsen… - Nature Reviews …, 2022 - nature.com
Alterations in protein structure may have profound effects on biological function. Analytical
techniques that permit characterization of proteins while maintaining their conformational …
techniques that permit characterization of proteins while maintaining their conformational …
Approaches to heterogeneity in native mass spectrometry
AD Rolland, JS Prell - Chemical reviews, 2021 - ACS Publications
Native mass spectrometry (MS) is aimed at preserving and determining the native structure,
composition, and stoichiometry of biomolecules and their complexes from solution after they …
composition, and stoichiometry of biomolecules and their complexes from solution after they …
Multi-attribute method (MAM): an emerging analytical workflow for biopharmaceutical characterization, batch release and cGMP purity testing at the peptide and intact …
S Millán-Martín, C Jakes, S Carillo… - Critical reviews in …, 2024 - Taylor & Francis
The rapid growth of biotherapeutic industry, with more and more complex molecules
entering the market, forces the need for advanced analytical platforms that can quickly and …
entering the market, forces the need for advanced analytical platforms that can quickly and …
New technologies bloom together for bettering cancer drug conjugates
Drug conjugates, including antibody-drug conjugates, are a step toward realizing Paul
Ehrlich's idea from over 100 years ago of a “magic bullet” for cancer treatment. Through …
Ehrlich's idea from over 100 years ago of a “magic bullet” for cancer treatment. Through …
Multiattribute monitoring of charge-based heterogeneity of recombinant monoclonal antibodies using 2D HIC-WCX-MS
Charged heterogeneity of monoclonal antibody (mAb) products is regarded as a critical
quality attribute (CQA) depending on its impact on the safety and efficacy profile of the …
quality attribute (CQA) depending on its impact on the safety and efficacy profile of the …
[HTML][HTML] Native LC–MS for capturing quality attributes of biopharmaceuticals on the intact protein level
F Füssl, L Strasser, S Carillo, J Bones - Current Opinion in Biotechnology, 2021 - Elsevier
Intact protein analysis by means of mass spectrometry has become a well-established
method for the characterization of biotherapeutics. However, due to the highly complex …
method for the characterization of biotherapeutics. However, due to the highly complex …
Native Proteomics by Capillary Zone Electrophoresis‐Mass Spectrometry
Native proteomics measures endogenous proteoforms and protein complexes under a near
physiological condition using native mass spectrometry (nMS) coupled with liquid‐phase …
physiological condition using native mass spectrometry (nMS) coupled with liquid‐phase …
Versatile, sensitive, and robust native LC–MS platform for intact mass analysis of protein drugs
Over the past several years, hyphenation of native (nondenaturing) liquid chromatography
(nLC) methods, such as size exclusion chromatography (SEC), ion exchange …
(nLC) methods, such as size exclusion chromatography (SEC), ion exchange …
Structural characterization and quantitation of ether-Linked glycerophospholipids in peroxisome biogenesis disorder tissue by ultraviolet photodissociation mass …
MS Blevins, SWJ Shields, W Cui, W Fallatah… - Analytical …, 2022 - ACS Publications
The biological impact of ether glycerophospholipids (GP) in peroxisomal disorders and other
diseases makes them significant targets as biomarkers for diagnostic assays or deciphering …
diseases makes them significant targets as biomarkers for diagnostic assays or deciphering …