Recent advances in targeting the “undruggable” proteins: from drug discovery to clinical trials
Undruggable proteins are a class of proteins that are often characterized by large, complex
structures or functions that are difficult to interfere with using conventional drug design …
structures or functions that are difficult to interfere with using conventional drug design …
Silk fibroin as a functional biomaterial for tissue engineering
Tissue engineering (TE) is the approach to combine cells with scaffold materials and
appropriate growth factors to regenerate or replace damaged or degenerated tissue or …
appropriate growth factors to regenerate or replace damaged or degenerated tissue or …
Genetically encoded fluorescent biosensors illuminate the spatiotemporal regulation of signaling networks
EC Greenwald, S Mehta, J Zhang - Chemical reviews, 2018 - ACS Publications
Cellular signaling networks are the foundation which determines the fate and function of
cells as they respond to various cues and stimuli. The discovery of fluorescent proteins over …
cells as they respond to various cues and stimuli. The discovery of fluorescent proteins over …
Silk fibroin-based biomaterials for tissue engineering applications
G Li, S Sun - Molecules, 2022 - mdpi.com
Tissue engineering (TE) involves the combination of cells with scaffolding materials and
appropriate growth factors in order to regenerate or replace damaged and degenerated …
appropriate growth factors in order to regenerate or replace damaged and degenerated …
Carbohydrate binding modules: diversity of domain architecture in amylases and cellulases from filamentous microorganisms
A Sidar, ED Albuquerque, GP Voshol… - … in Bioengineering and …, 2020 - frontiersin.org
Enzymatic degradation of abundant renewable polysaccharides such as cellulose and
starch is a field that has the attention of both the industrial and scientific community. Most of …
starch is a field that has the attention of both the industrial and scientific community. Most of …
Inhibition of RAS function through targeting an allosteric regulatory site
RAS GTPases are important mediators of oncogenesis in humans. However,
pharmacological inhibition of RAS has proved challenging. Here we describe a functionally …
pharmacological inhibition of RAS has proved challenging. Here we describe a functionally …
Synthetic single domain antibodies for the conformational trapping of membrane proteins
I Zimmermann, P Egloff, CAJ Hutter, FM Arnold… - elife, 2018 - elifesciences.org
Mechanistic and structural studies of membrane proteins require their stabilization in specific
conformations. Single domain antibodies are potent reagents for this purpose, but their …
conformations. Single domain antibodies are potent reagents for this purpose, but their …
Beyond natural antibodies: the power of in vitro display technologies
In vitro display technologies, best exemplified by phage and yeast display, were first
described for the selection of antibodies some 20 years ago. Since then, many antibodies …
described for the selection of antibodies some 20 years ago. Since then, many antibodies …
Applications of yeast surface display for protein engineering
GM Cherf, JR Cochran - Yeast Surface Display: Methods, Protocols, and …, 2015 - Springer
The method of displaying recombinant proteins on the surface of Saccharomyces cerevisiae
via genetic fusion to an abundant cell wall protein, a technology known as yeast surface …
via genetic fusion to an abundant cell wall protein, a technology known as yeast surface …
Affimer proteins are versatile and renewable affinity reagents
C Tiede, R Bedford, SJ Heseltine, G Smith, I Wijetunga… - Elife, 2017 - elifesciences.org
Molecular recognition reagents are key tools for understanding biological processes and are
used universally by scientists to study protein expression, localisation and interactions …
used universally by scientists to study protein expression, localisation and interactions …