Recombinational DNA repair is a universal aspect of DNA metabolism and is essential for
genomic integrity. It is a template-directed process that uses a second chromosomal copy …

Homologous recombination is a fundamental biological process. Biochemical
understanding of this process is most advanced for Escherichia coli. At least 25 gene …

CRISPR–Cas technologies have enabled programmable gene editing in eukaryotes and
prokaryotes. However, the leading Cas9 and Cas12a enzymes are limited in their ability to …

Mutations in the Artemis protein in humans result in hypersensitivity to DNA double-strand
break-inducing agents and absence of B and T lymphocytes (radiosensitive severe …

The structure, function and reactions of nucleic acids are central to molecular biology and
are crucial for the understanding of complex biological processes involved. Revised and …

Although homologous recombination and DNA repair phenomena in bacteria were initially
extensively studied without regard to any relationship between the two, it is now appreciated …

Eukaryotic cells possess several mechanisms for repairing double-stranded breaks in DNA.
One mechanism involves genetic recombination with an intact sister duplex. The recent …

The mouse Rad51 gene is a mammalian homologue of the Escherichia coli recA and yeast
RAD51 genes, both of which are involved in homologous recombination and DNA repair. To …

The generation of a double-strand break in the Saccharomyces cerevisiae genome is a
potentially catastrophic event that can induce cell-cycle arrest or ultimately result in loss of …

Recombination initiates at double-stranded DNA breaks and at single-stranded DNA gaps.
These DNA strand discontinuities can arise from DNA-damaging agents and from normal …