Bacillus thuringiensis insecticidal three-domain Cry toxins: mode of action, insect resistance and consequences for crop protection

L Pardo-López, M Soberón… - FEMS microbiology …, 2013 - academic.oup.com
Bacillus thuringiensis bacteria are insect pathogens that produce different Cry and Cyt toxins
to kill their hosts. Here we review the group of three-domain Cry (3d-Cry) toxins. Expression …

RNA interference in Lepidoptera: an overview of successful and unsuccessful studies and implications for experimental design

O Terenius, A Papanicolaou, JS Garbutt… - Journal of insect …, 2011 - Elsevier
Gene silencing through RNA interference (RNAi) has revolutionized the study of gene
function, particularly in non-model insects. However, in Lepidoptera (moths and butterflies) …

The next generation of insecticides: dsRNA is stable as a foliar‐applied insecticide

K San Miguel, JG Scott - Pest management science, 2016 - Wiley Online Library
BACKGROUND RNAi is a powerful tool used to study gene function. It also has been
hypothesized to be a promising new method for control of insect pests on crops, although …

Mechanisms of dsRNA uptake in insects and potential of RNAi for pest control: a review

H Huvenne, G Smagghe - Journal of insect physiology, 2010 - Elsevier
RNA interference already proved its usefulness in functional genomic research on insects,
but it also has considerable potential for the control of pest insects. For this purpose, the …

Control of coleopteran insect pests through RNA interference

JA Baum, T Bogaert, W Clinton, GR Heck… - Nature …, 2007 - nature.com
Commercial biotechnology solutions for controlling lepidopteran and coleopteran insect
pests on crops depend on the expression of Bacillus thuringiensis insecticidal proteins …

RNA interference: biology, mechanism, and applications

N Agrawal, PVN Dasaradhi, A Mohmmed… - Microbiology and …, 2003 - Am Soc Microbiol
Double-stranded RNA-mediated interference (RNAi) is a simple and rapid method of
silencing gene expression in a range of organisms. The silencing of a gene is a …

Prospects, challenges and current status of RNAi through insect feeding

N Kunte, E McGraw, S Bell, D Held… - Pest management …, 2020 - Wiley Online Library
RNA interference is a phenomenon in which the introduction of double‐stranded RNA
(dsRNA) into cells triggers the degradation of the complementary messenger RNA in a …

Diversity of Bacillus thuringiensis crystal toxins and mechanism of action

MJ Adang, N Crickmore, JL Jurat-Fuentes - Advances in insect physiology, 2014 - Elsevier
Parasporal crystals produced by Bacillus thuringiensis (Bt) bacteria are the main virulence
factors underlying Bt toxicity to insects. Parasporal crystals are composed primarily of Cry …

Ingested double-stranded RNAs can act as species-specific insecticides

S Whyard, AD Singh, S Wong - Insect biochemistry and molecular biology, 2009 - Elsevier
A serious shortcoming of many insecticides is that they can kill non-target species. To
address this issue, we harnessed the sequence specificity of RNA interference (RNAi) to …

Current models of the mode of action of Bacillus thuringiensis insecticidal crystal proteins: a critical review

V Vachon, R Laprade, JL Schwartz - Journal of invertebrate pathology, 2012 - Elsevier
Bacillus thuringiensis (Bt) Cry toxins constitute the active ingredient in the most widely used
biological insecticides and insect-resistant transgenic crops. A clear understanding of their …