Super-resolution microscopy demystified

L Schermelleh, A Ferrand, T Huser, C Eggeling… - Nature cell …, 2019 - nature.com
Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that
restricts the optical resolution to roughly 250 nm and was previously thought to be …

Fluorescence nanoscopy in cell biology

SJ Sahl, SW Hell, S Jakobs - Nature reviews Molecular cell biology, 2017 - nature.com
Fluorescence nanoscopy uniquely combines minimally invasive optical access to the
internal nanoscale structure and dynamics of cells and tissues with molecular detection …

MINFLUX nanometer-scale 3D imaging and microsecond-range tracking on a common fluorescence microscope

R Schmidt, T Weihs, CA Wurm, I Jansen… - Nature …, 2021 - nature.com
The recently introduced minimal photon fluxes (MINFLUX) concept pushed the resolution of
fluorescence microscopy to molecular dimensions. Initial demonstrations relied on custom …

Reversible 3D optical data storage and information encryption in photo-modulated transparent glass medium

Z Hu, X Huang, Z Yang, J Qiu, Z Song… - Light: Science & …, 2021 - nature.com
Transparent glass has been identified as a vital medium for three-dimensional (3D) optical
information storage and multi-level encryption. However, it has remained a challenge for …

Light in diagnosis, therapy and surgery

SH Yun, SJJ Kwok - Nature biomedical engineering, 2017 - nature.com
Light and optical techniques have made profound impacts on modern medicine, with
numerous lasers and optical devices currently being used in clinical practice to assess …

Super-resolution microscopy: a brief history and new avenues

K Prakash, B Diederich… - … Transactions of the …, 2022 - royalsocietypublishing.org
Super-resolution microscopy (SRM) is a fast-developing field that encompasses
fluorescence imaging techniques with the capability to resolve objects below the classical …

Photochromic fluorescent probe strategy for the super-resolution imaging of biologically important biomarkers

X Chai, HH Han, AC Sedgwick, N Li… - Journal of the …, 2020 - ACS Publications
Here, we report a β-galactosidase (β-Gal)-responsive photochromic fluorescent probe, NpG,
that was designed to prebind to human serum albumin (HSA) to form the probe/protein …

Supramolecular complex of photochromic diarylethene and cucurbit [7] uril: fluorescent photoswitching system for biolabeling and imaging

D Kim, A Aktalay, N Jensen, K Uno… - Journal of the …, 2022 - ACS Publications
Photoswitchable fluorophores─ proteins and synthetic dyes─ whose emission is reversibly
switched on and off upon illumination, are powerful probes for bioimaging, protein tracking …

Light-induced cell damage in live-cell super-resolution microscopy

S Wäldchen, J Lehmann, T Klein, S Van De Linde… - Scientific reports, 2015 - nature.com
Super-resolution microscopy can unravel previously hidden details of cellular structures but
requires high irradiation intensities to use the limited photon budget efficiently. Such high …

Switchable fluorophores for single-molecule localization microscopy

H Li, JC Vaughan - Chemical reviews, 2018 - ACS Publications
The past decade has witnessed an explosion in the use of super-resolution fluorescence
microscopy methods in biology and other fields. Single-molecule localization microscopy …