Nonequilibrium capillary electrophoresis of equilibrium mixtures− a single experiment reveals equilibrium and kinetic parameters of protein− DNA interactions
M Berezovski, SN Krylov - Journal of the American Chemical …, 2002 - ACS Publications
We introduce a novel electrophoretic method, nonequilibrium capillary electrophoresis of
equilibrium mixtures (NECEEM), and demonstrate its use for studying protein− DNA …
equilibrium mixtures (NECEEM), and demonstrate its use for studying protein− DNA …
Use of in vivo biotinylation to study protein–protein and protein–DNA interactions in mouse embryonic stem cells
In gene regulation, proteins function as members of protein complexes to recognize
chromosomal target DNA loci. In dissecting the pluripotent state in mouse embryonic stem …
chromosomal target DNA loci. In dissecting the pluripotent state in mouse embryonic stem …
Rapid agarose gel electrophoretic mobility shift assay for quantitating protein: RNA interactions
JA Ream, LK Lewis, KA Lewis - Analytical biochemistry, 2016 - Elsevier
Interactions between proteins and nucleic acids are frequently analyzed using
electrophoretic mobility shift assays (EMSAs). This technique separates bound protein …
electrophoretic mobility shift assays (EMSAs). This technique separates bound protein …
Rapid, sequential activation of mitogen-activated protein kinases and transcription factors precedes proinflammatory cytokine mRNA expression in spleens of mice …
HR Zhou, Z Islam, JJ Pestka - Toxicological sciences, 2003 - academic.oup.com
Since proinflammatory cytokine mRNA expression is induced within lymphoid tissue in vivo
by the trichothecene vomitoxin (VT) in a rapid (1–2 h) and transient (4–8 h) fashion, it was …
by the trichothecene vomitoxin (VT) in a rapid (1–2 h) and transient (4–8 h) fashion, it was …
Isolation and characterization of the E. coli membrane protein production strain Mutant56(DE3)
T Baumgarten, S Schlegel, S Wagner, M Löw… - Scientific reports, 2017 - nature.com
Membrane protein production is usually toxic to E. coli. However, using genetic screens
strains can be isolated in which the toxicity of membrane protein production is reduced …
strains can be isolated in which the toxicity of membrane protein production is reduced …
Induction of competing apoptotic and survival signaling pathways in the macrophage by the ribotoxic trichothecene deoxynivalenol
HR Zhou, Z Islam, JJ Pestka - Toxicological Sciences, 2005 - academic.oup.com
Deoxynivalenol (DON) and other ribotoxic trichothecenes cause immune stimulation and
suppression in leukocytes by upregulating gene expression and apoptosis, respectively …
suppression in leukocytes by upregulating gene expression and apoptosis, respectively …
Exploring strategies for the design of artificial transcription factors: targeting sites proximal to known regulatory regions for the induction of γ-globin expression and the …
T Gräslund, X Li, L Magnenat, M Popkov… - Journal of Biological …, 2005 - ASBMB
Artificial transcription factors can be engineered to interact with specific DNA sequences to
modulate endogenous gene expression within cells. A significant hurdle to implementation …
modulate endogenous gene expression within cells. A significant hurdle to implementation …
Regulation of poly (ADP-ribose) polymerase-1 (PARP-1) gene expression through the post-translational modification of Sp1: a nuclear target protein of PARP-1
K Zaniolo, S Desnoyers, S Leclerc, SL Guérin - BMC molecular biology, 2007 - Springer
Background Poly (ADP-ribose) polymerase-1 (PARP-1) is a nuclear enzyme that plays
critical functions in many biological processes, including DNA repair and gene transcription …
critical functions in many biological processes, including DNA repair and gene transcription …
P2Y2 receptor transcription is increased by NF-κB and stimulates cyclooxygenase-2 expression and PGE2 released by intestinal epithelial cells
E Degagné, DM Grbic, AA Dupuis… - The Journal of …, 2009 - journals.aai.org
Inflammatory stresses associated with inflammatory bowel diseases up-regulate P2Y 2
mRNA receptor expression in the human colon adenocarcinoma cell line Caco-2, the …
mRNA receptor expression in the human colon adenocarcinoma cell line Caco-2, the …
Recognition of duplex RNA by the deaminase domain of the RNA editing enzyme ADAR2
KJ Phelps, K Tran, T Eifler, AI Erickson… - Nucleic acids …, 2015 - academic.oup.com
Adenosine deaminases acting on RNA (ADARs) hydrolytically deaminate adenosines (A) in
a wide variety of duplex RNAs and misregulation of editing is correlated with human …
a wide variety of duplex RNAs and misregulation of editing is correlated with human …