[HTML][HTML] Guidelines for validation of qualitative real-time PCR methods

S Broeders, I Huber, L Grohmann, G Berben… - Trends in Food Science …, 2014 - Elsevier
Highlights•Guidelines for in-house and inter-laboratory validation of qualitative qPCR
methods.•Method acceptance and performance parameters to be evaluated.•Acceptance …

Plant 3'regulatory regions from mRNA-encoding genes and their uses to modulate expression

WS Bernardes, M Menossi - Frontiers in Plant Science, 2020 - frontiersin.org
Molecular biotechnology has made it possible to explore the potential of plants for different
purposes. The 3'regulatory regions have a great diversity of cis-regulatory elements directly …

Genome-Wide Characterization of the MLO Gene Family in Cannabis sativa Reveals Two Genes as Strong Candidates for Powdery Mildew Susceptibility

N Pépin, FO Hebert, DL Joly - Frontiers in Plant Science, 2021 - frontiersin.org
Cannabis sativa is increasingly being grown around the world for medicinal, industrial, and
recreational purposes. As in all cultivated plants, cannabis is exposed to a wide range of …

Two FAST multiplex real-time PCR reactions to assess the presence of genetically modified organisms in food

G Cottenet, C Blancpain, V Sonnard, PF Chuah - Food chemistry, 2019 - Elsevier
Methods to detect the presence of genetically modified organisms (GMOs) are evolving
constantly to comply with legislation and include new transgenic traits developed by …

The GMOseek matrix: a decision support tool for optimizing the detection of genetically modified plants

A Block, F Debode, L Grohmann, J Hulin, I Taverniers… - BMC …, 2013 - Springer
Background Since their first commercialization, the diversity of taxa and the genetic
composition of transgene sequences in genetically modified plants (GMOs) are constantly …

DNA-based methods for detection of genetically modified events in food and supply chain

G Randhawa, M Singh, P Sood - Current science, 2016 - JSTOR
Efficient detection strategies for genetically modified (GM) crops need to be in compliance
with regulatory frameworks and address consumer concerns. The present review describes …

Detection and identification of transgenic events by next generation sequencing combined with enrichment technologies

F Debode, J Hulin, B Charloteaux, W Coppieters… - Scientific reports, 2019 - nature.com
Next generation sequencing (NGS) is a promising tool for analysing the quality and safety of
food and feed products. The detection and identification of genetically modified organisms …

Development and Application of Loop-Mediated Isothermal Amplification Assays for Rapid Visual Detection of cry2Ab and cry3A Genes in Genetically-Modified …

F Li, W Yan, L Long, X Qi, C Li, S Zhang - International Journal of …, 2014 - mdpi.com
The cry2Ab and cry3A genes are two of the most important insect-resistant exogenous
genes and had been widely used in genetically-modified crops. To develop more effective …

Multiplex PCR system to track authorized and unauthorized genetically modified soybean events in food and feed

SB Park, HY Kim, JH Kim - Food Control, 2015 - Elsevier
A diverse range of genetic elements has been used to develop genetically modified
organisms (GMOs) over the last 18 years. Screening methods that target few elements, such …

Inter-laboratory studies for the validation of two singleplex (tE9 and pea lectin) and one duplex (pat/bar) real-time PCR methods for GMO detection

F Debode, I Huber, R Macarthur, PE Rischitor… - Food Control, 2017 - Elsevier
This paper presents the inter-laboratory studies for the successful validation of qualitative
real-time Polymerase Chain Reaction methods for the detection of Genetically Modified …