In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

Metabolic burden is defined by the influence of genetic manipulation and environmental
perturbations on the distribution of cellular resources. The rewiring of microbial metabolism …

The mammalian microbiome has many important roles in health and disease,, and genetic
engineering is enabling the development of microbial therapeutics and diagnostics,,,,. A key …

Cells use feedback regulation to ensure robust growth despite fluctuating demands for
resources and differing environmental conditions. However, the expression of foreign …

Ultrasound allows imaging at a much greater depth than optical methods, but existing
genetically encoded acoustic reporters for in vivo cellular imaging have been limited by poor …

Synthetic biology concerns the engineering of man‐made living biomachines from
standardized components that can perform predefined functions in a (self‐) controlled …

Despite recent advances in circuit engineering, the design of genetic networks in
mammalian cells is still painstakingly slow and fraught with inexplicable failures. Here, we …

A transition toward sustainable bio-based chemical production is important for green growth.
However, productivity and yield frequently decrease as large-scale microbial fermentation …

Yeast productivity in lignocellulosic ethanol fermentation is clearly impeded by stress.
Enhancing the robustness of xylose-fermenting yeast is important for improving …

In 1961, Jacob and Monod proposed the operon model of gene regulation. At the model's
core was the modular assembly of regulators, operators, and structural genes. To illustrate …