Chromatin in eukaryotic cells is a negatively charged polymer composed of DNA, histones,
and various associated proteins. Over the past ten years, our view of chromatin has shifted …

This article focuses on the role of the interchromatin compartment (IC) in shaping nuclear
landscapes. The IC is connected with nuclear pore complexes (NPCs) and harbors splicing …

Diverse cell types in tissues have distinct gene expression programs, chromatin states, and
nuclear architectures. To correlate such multimodal information across thousands of single …

Liquid–liquid phase separation (LLPS) is an important mechanism that helps explain the
membraneless compartmentalization of the nucleus. Because chromatin compaction and …

Due to its specificity, fluorescence microscopy has become a quintessential imaging tool in
cell biology. However, photobleaching, phototoxicity, and related artifacts continue to limit …

Although chromatin organization and dynamics play a critical role in gene transcription, how
they interplay remains unclear. To approach this issue, we investigated genome-wide …

Biomolecular condensates play a key role in organizing cellular reactions by concentrating a
specific set of biomolecules. However, whether condensate formation is accompanied by an …

Chromatin organization is crucial for regulating gene expression. Previously, we showed
that nucleosomes form groups, termed clutches. Clutch size correlated with the pluripotency …

Hoechst conjugates to fluorescent dyes are popular DNA stains for live-cell imaging, but the
relationship between their structure and performance remains elusive. This study of …

The cell nucleus is a compartment in which essential processes such as gene transcription
and DNA replication occur. Although the large amount of chromatin confined in the finite …