The promise and the challenges of cryo‐electron tomography
M Turk, W Baumeister - FEBS letters, 2020 - Wiley Online Library
Structural biologists have traditionally approached cellular complexity in a reductionist
manner in which the cellular molecular components are fractionated and purified before …
manner in which the cellular molecular components are fractionated and purified before …
Understanding the invisible hands of sample preparation for cryo-EM
Cryo-electron microscopy (cryo-EM) is rapidly becoming an attractive method in the field of
structural biology. With the exploding popularity of cryo-EM, sample preparation must evolve …
structural biology. With the exploding popularity of cryo-EM, sample preparation must evolve …
Topaz-Denoise: general deep denoising models for cryoEM and cryoET
Cryo-electron microscopy (cryoEM) is becoming the preferred method for resolving protein
structures. Low signal-to-noise ratio (SNR) in cryoEM images reduces the confidence and …
structures. Low signal-to-noise ratio (SNR) in cryoEM images reduces the confidence and …
Cryo-EM structure determination of small therapeutic protein targets at 3 Å-resolution using a rigid imaging scaffold
R Castells-Graells, K Meador… - Proceedings of the …, 2023 - National Acad Sciences
Cryoelectron microscopy (Cryo-EM) has enabled structural determination of proteins larger
than about 50 kDa, including many intractable by any other method, but it has largely failed …
than about 50 kDa, including many intractable by any other method, but it has largely failed …
Cryo-EM structure of SARS-CoV-2 ORF3a in lipid nanodiscs
Abstract SARS-CoV-2 ORF3a is a putative viral ion channel implicated in autophagy
inhibition, inflammasome activation and apoptosis. 3a protein and anti-3a antibodies are …
inhibition, inflammasome activation and apoptosis. 3a protein and anti-3a antibodies are …
Cryo-EM structure determination of small proteins by nanobody-binding scaffolds (Legobodies)
X Wu, TA Rapoport - … of the National Academy of Sciences, 2021 - National Acad Sciences
We describe a general method that allows structure determination of small proteins by single-
particle cryo-electron microscopy (cryo-EM). The method is based on the availability of a …
particle cryo-electron microscopy (cryo-EM). The method is based on the availability of a …
Cryo-electron microscopy methodology: current aspects and future directions
Cryo-electron microscopy (cryo-EM) has emerged as a powerful structure determination
technique. Its most prolific branch is single particle analysis (SPA), a method being used in a …
technique. Its most prolific branch is single particle analysis (SPA), a method being used in a …
Structural basis of nucleosome-dependent cGAS inhibition
JA Boyer, CJ Spangler, JD Strauss, AP Cesmat, P Liu… - Science, 2020 - science.org
Cyclic guanosine monophosphate (GMP)–adenosine monophosphate (AMP) synthase
(cGAS) recognizes cytosolic foreign or damaged DNA to activate the innate immune …
(cGAS) recognizes cytosolic foreign or damaged DNA to activate the innate immune …
Membranes under the magnetic lens: a dive into the diverse world of membrane protein structures using Cryo-EM
Membrane proteins are highly diverse in both structure and function and can, therefore,
present different challenges for structure determination. They are biologically important for …
present different challenges for structure determination. They are biologically important for …
Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution
The fast development of single-particle cryogenic electron microscopy (cryo-EM) has made it
more feasible to obtain the 3D structure of well-behaved macromolecules with a molecular …
more feasible to obtain the 3D structure of well-behaved macromolecules with a molecular …