Single-molecule fluorescence microscopy review: shedding new light on old problems

S Shashkova, MC Leake - Bioscience reports, 2017 - portlandpress.com
Fluorescence microscopy is an invaluable tool in the biosciences, a genuine workhorse
technique offering exceptional contrast in conjunction with high specificity of labelling with …

Single-molecule techniques in biophysics: a review of the progress in methods and applications

H Miller, Z Zhou, J Shepherd… - Reports on Progress …, 2017 - iopscience.iop.org
Single-molecule biophysics has transformed our understanding of biology, but also of the
physics of life. More exotic than simple soft matter, biomatter lives far from thermal …

Biophysical characterisation of DNA origami nanostructures reveals inaccessibility to intercalation binding sites

HL Miller, S Contera, AJM Wollman, A Hirst… - …, 2020 - iopscience.iop.org
Intercalation of drug molecules into synthetic DNA nanostructures formed through self-
assembled origami has been postulated as a valuable future method for targeted drug …

An automated image analysis framework for segmentation and division plane detection of single live Staphylococcus aureus cells which can operate at millisecond …

AJM Wollman, H Miller, S Foster, MC Leake - Physical biology, 2016 - iopscience.iop.org
Staphylococcus aureus is an important pathogen, giving rise to antimicrobial resistance in
cell strains such as Methicillin Resistant S. aureus (MRSA). Here we report an image …

Transcription factors in eukaryotic cells can functionally regulate gene expression by acting in oligomeric assemblies formed from an intrinsically disordered protein …

MC Leake - Transcription, 2018 - Taylor & Francis
High-speed single-molecule fluorescence microscopy in vivo shows that transcription factors
in eukaryotes can act in oligomeric clusters mediated by molecular crowding and …

Systems biophysics: Single-molecule optical proteomics in single living cells

S Shashkova, MC Leake - Current Opinion in Systems Biology, 2018 - Elsevier
How does the interplay between biomolecules result in the emergence of cellular complexity
at higher length scales? This interplay in even simple biological processes is often too …

Magneto-optical tweezers with super-resolution fluorescence microscopy

Z Zhou - 2017 - etheses.whiterose.ac.uk
This thesis describes the design, construction and application of a novel magneto-optical
tweezers with super-resolution fluorescence microscopy for manipulation, force/torque …

[PDF][PDF] Single-molecule fluorescence microscopy: shedding new light on old problems

S Shashkova, MC Leake - researchgate.net
Fluorescence microscopy is an invaluable tool in the biosciences, a genuine workhorse
technique offering exceptional contrast in conjunction with high specificity of labelling with …

Automated image segmentation and division plane detection in single live Staphylococcus aureus cells

AJM Wollman, H Miller, S Foster, MC Leake - arXiv preprint arXiv …, 2016 - arxiv.org
Staphylococcus aureus is a coccal bacterium, which divides by binary fission. After division
the cells remain attached giving rise to small clusters, with a characteristic'bunch of grapes' …