pH-Induced Molten Globule State of Rhizopus niveus Lipase is More Resistant Against Thermal and Chemical Denaturation Than Its Native State

G Rabbani, E Ahmad, N Zaidi, S Fatima… - Cell Biochemistry and …, 2012 - Springer
Here, we have characterized four pH-dependent states: alkaline state,“B”(pH 9.0), native
state,“N”(pH 7.4), acid-induced state,“A”(pH 2.2) and molten globule state,“MG”(pH 1.8) of …

Thermal inactivation of glucose oxidase: Mechanism and stabilization using additives

MD Gouda, SA Singh, AGA Rao, MS Thakur… - Journal of Biological …, 2003 - ASBMB
Thermal inactivation of glucose oxidase (GOD; β-d-glucose: oxygen oxidoreductase), from
Aspergillus niger, followed first order kinetics both in the absence and presence of additives …

Periodically Patterned Au-TiO2 Heterostructures for Photoelectrochemical Sensor

L Guo, Z Li, K Marcus, S Navarro, K Liang, L Zhou… - ACS …, 2017 - ACS Publications
Periodically patterned Au nanorods in TiO2 nanocavities (Au NRs@ TiO2) were fabricated
via magnetron sputtering followed by a thermal dewetting process. This innovative Au …

Apo-metallothionein-3 cooperatively forms tightly compact structures under physiological conditions

AT Yuan, NC Korkola, MJ Stillman - Journal of Biological Chemistry, 2023 - ASBMB
Metallothioneins (MTs) are essential mammalian metal chaperones. MT isoform 1 (MT1) is
expressed in the kidneys and isoform 3 (MT3) is expressed in nervous tissue. For MTs, the …

Distant Phe345 mutation compromises the stability and activity of Mycobacterium tuberculosis isocitrate lyase by modulating its structural flexibility

H Shukla, R Shukla, A Sonkar, T Pandey, T Tripathi - Scientific reports, 2017 - nature.com
Isocitrate lyase (ICL), a potential anti-tubercular drug target, catalyzes the first step of the
glyoxylate shunt. In the present investigation, we studied the conformational flexibility of …

On the molecular mechanism of ion specific Hofmeister series

WJ Xie, CW Liu, LJ Yang, YQ Gao - Science China Chemistry, 2014 - Springer
Hofmeister series ranks the ability of salt ions in influencing a variety of properties and
processes in aqueous solutions. In this review, we reexamine how these ions and some …

Severe acute respiratory syndrome coronavirus 3C-like proteinase N terminus is indispensable for proteolytic activity but not for enzyme dimerization: biochemical and …

S Chen, L Chen, J Tan, J Chen, L Du, T Sun… - Journal of Biological …, 2005 - ASBMB
Severe acute respiratory syndrome (SARS) coronavirus is a novel human coronavirus and is
responsible for SARS infection. SARS coronavirus 3C-like proteinase (SARS 3CL pro) plays …

Live diatom silica immobilization of multimeric and redox-active enzymes

VC Sheppard, A Scheffel, N Poulsen… - Applied and …, 2012 - Am Soc Microbiol
Living organisms are adept in forming inorganic materials (biominerals) with unique
structures and properties that exceed the capabilities of engineered materials. Biomimetic …

Immobilization of glucose oxidase on bioinspired polyphenol coatings as a high-throughput glucose assay platform

S Sunoqrot, A Al-Hadid, A Manasrah, R Khnouf… - RSC …, 2021 - pubs.rsc.org
Glucose oxidase (GOx) is an enzyme with important industrial and biochemical applications,
particularly in glucose detection. Here we leveraged the oxidative self-polymerization …

A resettable and reprogrammable keypad lock based on electrochromic Prussian blue films and biocatalysis of immobilized glucose oxidase in a bipolar electrode …

X Yu, J Liang, T Yang, M Gong, D Xi, H Liu - Biosensors and Bioelectronics, 2018 - Elsevier
Herein, a resettable and reprogrammable biomolecular keypad lock on the basis of a closed
bipolar electrode (BPE) system was established. In this system, one ITO electrode with …