CRISPR technologies and the search for the PAM-free nuclease
D Collias, CL Beisel - Nature communications, 2021 - nature.com
The ever-expanding set of CRISPR technologies and their programmable RNA-guided
nucleases exhibit remarkable flexibility in DNA targeting. However, this flexibility comes with …
nucleases exhibit remarkable flexibility in DNA targeting. However, this flexibility comes with …
Precision genome editing using cytosine and adenine base editors in mammalian cells
Genome editing has transformed the life sciences and has exciting prospects for use in
treating genetic diseases. Our laboratory developed base editing to enable precise and …
treating genetic diseases. Our laboratory developed base editing to enable precise and …
CRISPR-Cas tools and their application in genetic engineering of human stem cells and organoids
D Hendriks, H Clevers, B Artegiani - Cell stem cell, 2020 - cell.com
CRISPR-Cas technology has revolutionized biological research and holds great therapeutic
potential. Here, we review CRISPR-Cas systems and their latest developments with an …
potential. Here, we review CRISPR-Cas systems and their latest developments with an …
Improved genome editing by an engineered CRISPR-Cas12a
CRISPR-Cas12a is an RNA-guided, programmable genome editing enzyme found within
bacterial adaptive immune pathways. Unlike CRISPR-Cas9, Cas12a uses only a single …
bacterial adaptive immune pathways. Unlike CRISPR-Cas9, Cas12a uses only a single …
Discovery of diverse CRISPR-Cas systems and expansion of the genome engineering toolbox
CRISPR systems mediate adaptive immunity in bacteria and archaea through diverse
effector mechanisms and have been repurposed for versatile applications in therapeutics …
effector mechanisms and have been repurposed for versatile applications in therapeutics …
CRISPR-Cas-mediated transcriptional modulation: The therapeutic promises of CRISPRa and CRISPRi
L Bendixen, TI Jensen, RO Bak - Molecular Therapy, 2023 - cell.com
The CRISPR-Cas system is commonly known for its ability to cleave DNA in a
programmable manner, which has democratized gene editing and facilitated recent …
programmable manner, which has democratized gene editing and facilitated recent …
CRISPR-Cas12a (Cpf1): a versatile tool in the plant genome editing tool box for agricultural advancement
A Bandyopadhyay, N Kancharla, VS Javalkote… - Frontiers in plant …, 2020 - frontiersin.org
Global population is predicted to approach 10 billion by 2050, an increase of over 2 billion
from today. To meet the demands of growing, geographically and socio-economically …
from today. To meet the demands of growing, geographically and socio-economically …
The design and application of DNA-editing enzymes as base editors
KL Rallapalli, AC Komor - Annual review of biochemistry, 2023 - annualreviews.org
DNA-editing enzymes perform chemical reactions on DNA nucleobases. These reactions
can change the genetic identity of the modified base or modulate gene expression. Interest …
can change the genetic identity of the modified base or modulate gene expression. Interest …
Recent advances in CRISPR-based genome editing technology and its applications in cardiovascular research
ZH Li, J Wang, JP Xu, J Wang, X Yang - Military Medical Research, 2023 - Springer
The rapid development of genome editing technology has brought major breakthroughs in
the fields of life science and medicine. In recent years, the clustered regularly interspaced …
the fields of life science and medicine. In recent years, the clustered regularly interspaced …
Past, present, and future of CRISPR genome editing technologies
Genome editing has been a transformative force in the life sciences and human medicine,
offering unprecedented opportunities to dissect complex biological processes and treat the …
offering unprecedented opportunities to dissect complex biological processes and treat the …