In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

A detailed knowledge of the complex processes that make cells and organisms alive is
fundamental in order to understand diseases and to develop novel drugs and therapeutic …

It has long been axiomatic that a protein's structure determines its function. Intrinsically
disordered proteins (IDPs) and disordered protein regions (IDRs) defy this structure …

NMR spectroscopy has been applied to cells and tissues analysis since its beginnings, as
early as 1950. We have attempted to gather here in a didactic fashion the broad diversity of …

Classical structural biology approaches allow structural characterization of biological
macromolecules in vitro, far from their physiological context. Nowadays, thanks to the wealth …

Studying biomolecules at atomic resolution in their native environment is the ultimate aim of
structural biology. We investigated the bacterial type IV secretion system core complex …

Ever since scientists realized that cells are the basic building blocks of all life, they have
been developing tools to look inside them to reveal the architectures and mechanisms that …

NMR has the resolution and specificity to determine atomic-level protein structures of
isotopically labeled proteins in complex environments, and with the sensitivity gains …

The content of mitochondrial proteome is maintained through two highly dynamic processes,
the influx of newly synthesized proteins from the cytosol and the protein degradation …

Mutations in the superoxide dismutase 1 (SOD1) gene are related to familial cases of
amyotrophic lateral sclerosis (fALS). Here we exploit in-cell NMR to characterize the protein …