Type II polyketide synthases (PKSs) are protein assemblies, encoded by biosynthetic gene
clusters in microorganisms, that manufacture structurally complex and pharmacologically …

In recent years, the mammalian mitochondrial protein complex for iron–sulfur cluster
assembly has been the focus of important studies. This is partly because of its high degree …

Engineering microbial biosynthetic pathways represents a compelling route to gain access
to expanded chemical diversity. Carrier proteins (CPs) play a central role in biosynthesis, but …

Non-ribosomal peptide synthetases (NRPSs) generate chemically complex compounds and
their modular architecture suggests that changing their domain organization can predictably …

Enzyme assemblies such as type II polyketide synthases (PKSs) produce a wide array of
bioactive secondary metabolites. While the molecules produced by type II PKSs have found …

The strategic redesign of microbial biosynthetic pathways is a compelling route to access
molecules of diverse structure and function in a potentially environmentally sustainable …

The ability to produce new molecules of potential pharmaceutical relevance via
combinatorial biosynthesis hinges on improving our understanding of acyl-carrier protein …

E. coli expression system is the method of choice to obtain high yields of a pure protein.
However, there is always a chance that an overexpressed protein shares structural or …

Results Purification of holo-ACPs and FabF. AcpP, ACT ACP and FabF were expressed as
His6-tagged constructs in BAP1 10 or BL21-DE3 competent cells. We originally intended to …

Results Characterization of chain sequestration behavior. For proof-ofconcept experiments,
we collected data from three acyl carrier proteins (ACPs) for which chain sequestration …