[HTML][HTML] Strategies to overcome the main challenges of the use of CRISPR/Cas9 as a replacement for cancer therapy
CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats-associated protein
9) shows the opportunity to treat a diverse array of untreated various genetic and …
9) shows the opportunity to treat a diverse array of untreated various genetic and …
Computational tools and resources for CRISPR/Cas genome editing
The past decade has witnessed a rapid evolution in identifying more versatile clustered
regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) …
regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) …
Programmed genome editing by a miniature CRISPR-Cas12f nuclease
Z Wu, Y Zhang, H Yu, D Pan, Y Wang, Y Wang… - Nature chemical …, 2021 - nature.com
The RNA-guided CRISPR-associated (Cas) nucleases are versatile tools for genome editing
in various organisms. The large sizes of the commonly used Cas9 and Cas12a nucleases …
in various organisms. The large sizes of the commonly used Cas9 and Cas12a nucleases …
CRISPResso2 provides accurate and rapid genome editing sequence analysis
To the Editor—The field of genome editing is advancing rapidly1, most recently exemplified
by the advent of base editors that enable changing single nucleotides in a predictable …
by the advent of base editors that enable changing single nucleotides in a predictable …
Evolutionary mining and functional characterization of TnpB nucleases identify efficient miniature genome editors
G Xiang, Y Li, J Sun, Y Huo, S Cao, Y Cao, Y Guo… - Nature …, 2024 - nature.com
As the evolutionary ancestor of Cas12 nuclease, the transposon (IS200/IS605)-encoded
TnpB proteins act as compact RNA-guided DNA endonucleases. To explore their …
TnpB proteins act as compact RNA-guided DNA endonucleases. To explore their …
Plant gene editing through de novo induction of meristems
Plant gene editing is typically performed by delivering reagents such as Cas9 and single
guide RNAs to explants in culture. Edited cells are then induced to differentiate into whole …
guide RNAs to explants in culture. Edited cells are then induced to differentiate into whole …
Application of prime editing to the correction of mutations and phenotypes in adult mice with liver and eye diseases
H Jang, DH Jo, CS Cho, JH Shin, JH Seo… - Nature biomedical …, 2022 - nature.com
The use of prime editing—a gene-editing technique that induces small genetic changes
without the need for donor DNA and without causing double strand breaks—to correct …
without the need for donor DNA and without causing double strand breaks—to correct …
Human glioblastoma arises from subventricular zone cells with low-level driver mutations
Glioblastoma (GBM) is a devastating and incurable brain tumour, with a median overall
survival of fifteen months,. Identifying the cell of origin that harbours mutations that drive …
survival of fifteen months,. Identifying the cell of origin that harbours mutations that drive …
A biodegradable nanocapsule delivers a Cas9 ribonucleoprotein complex for in vivo genome editing
Delivery technologies for the CRISPR-Cas9 (CRISPR, clustered regularly interspaced short
palindromic repeats) gene editing system often require viral vectors, which pose safety …
palindromic repeats) gene editing system often require viral vectors, which pose safety …
Multiplexed heritable gene editing using RNA viruses and mobile single guide RNAs
EE Ellison, U Nagalakshmi, ME Gamo, P Huang… - Nature plants, 2020 - nature.com
An in planta gene editing approach was developed wherein Cas9 transgenic plants are
infected with an RNA virus that expresses single guide RNAs (sgRNAs). The sgRNAs are …
infected with an RNA virus that expresses single guide RNAs (sgRNAs). The sgRNAs are …