Reference gene selection for qRT-PCR analysis of season- and tissue-specific gene expression profiles in the honey bee Apis mellifera

JH Jeon, KH Moon, YH Kim, YH Kim - Scientific Reports, 2020 - nature.com
Honey bees are both important pollinators and model insects due to their highly developed
sociality and colony management. To better understand the molecular mechanisms …

Effects of dietary glutamine on survival, growth performance, activities of digestive enzyme, antioxidant status and hypoxia stress resistance of half-smooth tongue sole …

J Liu, K Mai, W Xu, Y Zhang, H Zhou, Q Ai - Aquaculture, 2015 - Elsevier
A 30-day feeding trial was conducted to evaluate the effects of dietary glutamine
supplementation on survival, growth performance, activities of digestive enzyme, antioxidant …

Development of a gene-deleted live attenuated candidate vaccine against fish virus (ISKNV) with low pathogenicity and high protection

R Zeng, W Pan, Y Lin, J He, Z Luo, Z Li, S Weng, J He… - Iscience, 2021 - cell.com
Aquaculture provides important food, nutrition, and income sources for humans. However,
aquaculture industry is seriously threatened by viral diseases. Infectious spleen and kidney …

[HTML][HTML] Validation of stable reference genes in peripheral blood mononuclear cells for expression studies involving vector-borne haemoparasitic diseases in bovines

R Kaur, S Ahlawat, V Choudhary, A Kumari… - Ticks and Tick-borne …, 2023 - Elsevier
Normalization of gene expression data using appropriate reference genes is critical to
diminish any technical bias in an experiment involving quantitative real-time PCR (qPCR) …

Evaluation of candidate reference genes for quantitative RTqPCR analysis in goldfish (Carassius auratus L.) in healthy and CyHV-2 infected fish

A Dharmaratnam, A Sudhagar… - Veterinary Immunology …, 2021 - Elsevier
The accuracy of quantitative real time PCR (RTqPCR) can be attained only when a suitable
reference gene is used. The gene expression for a particular gene may vary within different …

Investigation of the effects of estrogen on skeletal gene expression during zebrafish larval head development

EP Ahi, BS Walker, CS Lassiter, ZO Jónsson - PeerJ, 2016 - peerj.com
The development of craniofacial skeletal structures requires well-orchestrated tissue
interactions controlled by distinct molecular signals. Disruptions in normal function of these …

Evaluation of qPCR reference genes in GH-overexpressing transgenic zebrafish (Danio rerio)

GT Rassier, TLR Silveira, MH Remião, LO Daneluz… - Scientific Reports, 2020 - nature.com
Reference genes (RGs) must have a stable expression in tissues in all experimental
conditions to normalize real-time quantitative reverse transcription PCR (qRT-PCR) data …

Validation of housekeeping genes for gene expression profiling in fish: a necessity

M Faheem, S Khaliq - Critical Reviews™ in Eukaryotic Gene …, 2019 - dl.begellhouse.com
Real-time polymerase chain reaction (qPCR) is a reliable tool for gene expression analysis
in various organisms. Its use in aquaculture and study of fish physiology is increasing day by …

Screening and stability analysis of reference genes for gene expression normalization in hybrid yellow catfish (Pelteobagrus fulvidraco♀× Pelteobagrus vachelli♂) …

C Guo, Z Zhang, M Zhang, G Guo, G Yu… - Aquaculture …, 2023 - Wiley Online Library
In this study, we screened the expression stability of six reference genes (18S rRNA, β-actin,
GAPDH, EF1a, B2M, and HPRT1) in hybrid yellow catfish (n= 6), considering the SBM …

Identifying suitable reference genes for developing and injured mouse CNS tissues

D Xu, A Liu, X Wang, M Zhang, Z Zhang… - Developmental …, 2018 - Wiley Online Library
Accurate quantification of gene expression is fundamental for understanding the molecular,
genetic and functional bases of tissue development and diseases. Quantitative real‐time …