Nucleic acid amplification technologies (NAATs) have become fundamental tools in
molecular diagnostics, due to their ability to detect small amounts of target molecules. Since …

DNA analysis has seen an incredible development in terms of instrumentation, assays and
applications over the last years. Massively parallel sequencing (MPS) and digital PCR are …

Recent long-read assemblies often exceed the quality and completeness of available
reference genomes, making validation challenging. Here we present Merqury, a novel tool …

Next-generation sequencing (NGS) is the method of choice when large numbers of
sequences have to be obtained. While the technique is widely applied, varying error rates …

BACKGROUND First introduced in 2006, recombinase polymerase amplification (RPA) has
stirred great interest, as evidenced by 75 publications as of October 2015, with 56 of them …

Circulating tumor DNA (ctDNA) is a promising biomarker for noninvasive assessment of
cancer burden, but existing ctDNA detection methods have insufficient sensitivity or patient …

An ordered draft sequence of the 17-gigabase hexaploid bread wheat (Triticum aestivum)
genome has been produced by sequencing isolated chromosome arms. We have annotated …

We describe a rapid target enrichment method for next-generation sequencing, termed
anchored multiplex PCR (AMP), that is compatible with low nucleic acid input from formalin …

High-throughput sequencing, also known as next-generation sequencing (NGS), has
revolutionized genomic research. In recent years, NGS technology has steadily improved …

Background DNA sequencing technologies deviate from the ideal uniform distribution of
reads. These biases impair scientific and medical applications. Accordingly, we have …