Light-sheet fluorescence microscopy has transformed our ability to visualize and
quantitatively measure biological processes rapidly and over long time periods. In this …

Super-resolution imaging has rapidly emerged as an optical microscopy technique, offering
advantages of high optical resolution over the past two decades; achieving improved …

Quantification of microglial activation through morphometric analysis has long been a staple
of the neuroimmunologist's toolkit. Microglial morphological phenomics can be conducted …

Rapid, high-resolution volumetric imaging without moving heavy objectives or disturbing
delicate samples remains challenging. Pupil-matched remote focusing offers a promising …

Projection imaging accelerates volumetric interrogation in fluorescence microscopy, but for
multi-cellular samples, the resulting images may lack contrast, as many structures and haze …

Breast cancer (BC) is the most common tumor worldwide and requires crucial molecular
typing for treatment and prognosis assessment. Currently, approaches like pathological …

Oblique plane microscopy (OPM) directly captures object information in a plane tilted from
the focal plane of the objective lens without the need for slow z-stack acquisition. This …

Fundamental properties of light unavoidably impose features on images collected using
fluorescence microscopes. Accounting for these features is often critical in quantitatively …

The background light from out-of-focus planes hinders resolution enhancement in structured
illumination microscopy when observing volumetric samples. Here we used selective plane …

Fluorescence microscopic imaging is essentially a convolution process distorted by random
noise, limiting critical parameters such as imaging speed, duration, and resolution. Though …