MIQE: a step toward more robust and reproducible quantitative PCR
SA Bustin, CT Wittwer - Clinical chemistry, 2017 - academic.oup.com
Featured Article: Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, et al.
The MIQE guidelines: minimum information for publication of quantitative realtime PCR …
The MIQE guidelines: minimum information for publication of quantitative realtime PCR …
Improving the quality of quantitative polymerase chain reaction experiments: 15 years of MIQE
SA Bustin - Molecular aspects of medicine, 2024 - Elsevier
The quantitative polymerase chain reaction (qPCR) is fundamental to molecular biology. It is
not just a laboratory technique, qPCR is a bridge between research and clinical practice. Its …
not just a laboratory technique, qPCR is a bridge between research and clinical practice. Its …
Two clade a phosphatase 2Cs expressed in guard cells physically interact with abscisic acid signaling components to induce stomatal closure in rice
MK Min, EH Choi, JA Kim, IS Yoon, S Han, Y Lee… - Rice, 2019 - Springer
Background The core ABA signaling components functioning in stomatal closure/opening,
namely ABA receptors, phosphatases, SnRK2s and SLAC1, are well characterized in …
namely ABA receptors, phosphatases, SnRK2s and SLAC1, are well characterized in …
Sexual dimorphic effects of igf1 deficiency on metabolism in zebrafish
N Zeng, J Bao, TT Shu, C Shi, G Zhai, X Jin… - Frontiers in …, 2022 - frontiersin.org
Insulin-like growth factor 1 (IGF1) is an essential effector of the growth hormone (GH)/IGF1
axis for somatic growth regulation in mammals. However, its functions have not been …
axis for somatic growth regulation in mammals. However, its functions have not been …
Amplification efficiency and thermal stability of qPCR instrumentation: Current landscape and future perspectives
KR Rogers‑Broadway… - Experimental and …, 2015 - spandidos-publications.com
Quantitative polymerase chain reaction (qPCR) is a method of amplifying and detecting
small samples of genetic material in real time and is in routine use across many laboratories …
small samples of genetic material in real time and is in routine use across many laboratories …
Determination of the panel of reference genes for quantitative real-time PCR in fetal and adult rat intestines
X Lu, Y Liu, D Zhang, K Liu, Q Wang, H Wang - Reproductive Toxicology, 2021 - Elsevier
In quantitative real-time PCR (qRT-PCR) detection, the stability of reference genes varies
with different organs, tissue locations, sex and developmental stages. This study aimed to …
with different organs, tissue locations, sex and developmental stages. This study aimed to …
Selection and verification of the combination of reference genes for RT-qPCR analysis in rat adrenal gland development
X Xia, Y Liu, L Liu, Y Chen, H Wang - The Journal of Steroid Biochemistry …, 2021 - Elsevier
Quantitative reverse transcription polymerase chain reaction (RT-qPCR) is commonly used
for gene expression analysis, and the accuracy of its results depends greatly on chosen …
for gene expression analysis, and the accuracy of its results depends greatly on chosen …
Identification and validation of reference genes for RT-qPCR analysis in fetal rat pancreas
Y Dai, H Kou, X Guo, Z Gong, H Liu, Y Liu… - Reproductive …, 2021 - Elsevier
The choice of reference gene is crucial for quantitative reverse transcriptase-polymerase
chain reaction (RT-qPCR) assay. To screen and determine the suitable reference genes in …
chain reaction (RT-qPCR) assay. To screen and determine the suitable reference genes in …
Selenium mediated host plant–mite conflict: defense and adaptation
Z Xu, CC Qi, M Zhang, J Zhu, J Hu… - Pest Management …, 2021 - Wiley Online Library
BACKGROUND Selenium has shown effectiveness in protecting plants from herbivores.
However, some insects have evolved adaptability to selenium. RESULTS Selenium …
However, some insects have evolved adaptability to selenium. RESULTS Selenium …
[HTML][HTML] Implementation of Rapid Nucleic Acid Amplification Based on the Super Large Thermoelectric Cooler Rapid Temperature Rise and Fall Heating Module
J Cheng, E Zhang, R Sun, K Zhang, F Zhang, J Zhao… - Biosensors, 2024 - mdpi.com
In the rapid development of molecular biology, nucleic acid amplification detection
technology has received more and more attention. The traditional polymerase chain reaction …
technology has received more and more attention. The traditional polymerase chain reaction …