Synthetic messenger RNA (mRNA) has been focused on as an emerging application for
mRNA-based therapies and vaccinations. Recently, synthetic circular RNAs (circRNAs) …

The bottom-up assembly of biological components in synthetic biology has contributed to a
better understanding of natural phenomena and the development of new technologies for …

RNA-based synthetic genetic circuits provide an alternative for traditional transcription-
based circuits in applications where genomic integration is to be avoided. Incorporating …

Synthetic biological circuits are designed to regulate gene expressions to control cell
function. To date, these circuits often use DNA-delivery methods, which may lead to random …

Synthetic messenger RNA (mRNA) tools often use pseudouridine and 5-methyl cytidine as
substitutions for uridine and cytidine to avoid the immune response and cytotoxicity induced …

The efficiency of pluripotent stem cell differentiation is highly variable, often resulting in
heterogeneous populations that contain undifferentiated cells. Here we developed a …

MicroRNAs (miRNA or miR) are short noncoding RNA of about 21–23 nucleotides that play
critical roles in multiple aspects of biological processes by mediating translational …

For regenerative cell therapies using pluripotent stem cell (PSC)-derived cells, large
quantities of purified cells are required. Magnetic-activated cell sorting (MACS) is a powerful …

Synthetic RNA-based gene circuits enable sophisticated gene regulation without the risk of
insertional mutagenesis. While various RNA binding proteins have been used for …

The photo-regulation of transgene expression is one effective approach in mammalian
synthetic biology due to its high spatial and temporal resolution. While DNAs are mainly …