Affinity‐purification coupled to mass spectrometry: Basic principles and strategies
WH Dunham, M Mullin, AC Gingras - Proteomics, 2012 - Wiley Online Library
Identifying the interactions established by a protein of interest can be a critical step in
understanding its function. This is especially true when an unknown protein of interest is …
understanding its function. This is especially true when an unknown protein of interest is …
Roles of COP9 signalosome in cancer
The constitutive photomorphogenesis 9 signalosome (COP9 or CSN) is an evolutionarily
conserved multiprotein complex found in plants and animals. Because of the homology …
conserved multiprotein complex found in plants and animals. Because of the homology …
Structural basis of indisulam-mediated RBM39 recruitment to DCAF15 E3 ligase complex
DE Bussiere, L Xie, H Srinivas, W Shu, A Burke… - Nature Chemical …, 2020 - nature.com
The anticancer agent indisulam inhibits cell proliferation by causing degradation of RBM39,
an essential mRNA splicing factor. Indisulam promotes an interaction between RBM39 and …
an essential mRNA splicing factor. Indisulam promotes an interaction between RBM39 and …
Structural complementarity facilitates E7820-mediated degradation of RBM39 by DCAF15
The investigational drugs E7820, indisulam and tasisulam (aryl-sulfonamides) promote the
degradation of the splicing factor RBM39 in a proteasome-dependent mechanism. While the …
degradation of the splicing factor RBM39 in a proteasome-dependent mechanism. While the …
Structural basis and kinetic pathway of RBM39 recruitment to DCAF15 by a sulfonamide molecular glue E7820
X Du, OA Volkov, RM Czerwinski, HL Tan, C Huerta… - Structure, 2019 - cell.com
E7820 and indisulam are two examples of aryl sulfonamides that recruit RBM39 to Rbx-Cul4-
DDA1-DDB1-DCAF15 E3 ligase complex, leading to its ubiquitination and degradation by …
DDA1-DDB1-DCAF15 E3 ligase complex, leading to its ubiquitination and degradation by …
Dynamics of cullin-RING ubiquitin ligase network revealed by systematic quantitative proteomics
Dynamic reorganization of signaling systems frequently accompanies pathway
perturbations, yet quantitative studies of network remodeling by pathway stimuli are lacking …
perturbations, yet quantitative studies of network remodeling by pathway stimuli are lacking …
Aryl sulfonamides degrade RBM39 and RBM23 by recruitment to CRL4-DCAF15
Indisulam and related sulfonamides recruit the splicing factor RBM39 to the CRL4-DCAF15
E3 ubiquitin ligase, resulting in RBM39 ubiquitination and degradation. Here, we used a …
E3 ubiquitin ligase, resulting in RBM39 ubiquitination and degradation. Here, we used a …
Targeted Degradation of Abscisic Acid Receptors Is Mediated by the Ubiquitin Ligase Substrate Adaptor DDA1 in Arabidopsis
ML Irigoyen, E Iniesto, L Rodriguez, MI Puga… - The Plant …, 2014 - academic.oup.com
Abstract CULLIN4-RING E3 ubiquitin ligases (CRL4s) regulate key developmental and
stress responses in eukaryotes. Studies in both animals and plants have led to the …
stress responses in eukaryotes. Studies in both animals and plants have led to the …
Structural basis for a reciprocal regulation between SCF and CSN
RI Enchev, DC Scott, PCA da Fonseca, A Schreiber… - Cell reports, 2012 - cell.com
Summary Skp1-Cul1-Fbox (SCF) E3 ligases are activated by ligation to the ubiquitin-like
protein Nedd8, which is reversed by the deneddylating Cop9 signalosome (CSN). However …
protein Nedd8, which is reversed by the deneddylating Cop9 signalosome (CSN). However …
The CUL4B‐based E3 ubiquitin ligase regulates mitosis and brain development by recruiting phospho‐specific DCAFs
A Stier, S Gilberto, WI Mohamed, LN Royall… - The EMBO …, 2023 - embopress.org
The paralogs CUL4A and CUL4B assemble cullin‐RING E3 ubiquitin ligase (CRL)
complexes regulating multiple chromatin‐associated cellular functions. Although they are …
complexes regulating multiple chromatin‐associated cellular functions. Although they are …