Real-time PCR is the method of choice in many laboratories for diagnostic and food
applications. This technology merges the polymerase chain reaction chemistry with the use …

Nearly 40 years have elapsed since the invention of the PCR, with its extremely sensitive
and specific ability to detect nucleic acids via in vitro enzyme-mediated amplification. In turn …

Abstract The Alzheimer Disease Genetics Consortium (ADGC) performed a genome-wide
association study of late-onset Alzheimer disease using a three-stage design consisting of a …

Use of the real-time polymerase chain reaction (PCR) to amplify cDNA products reverse
transcribed from mRNA is on the way to becoming a routine tool in molecular biology to …

Real-time reverse transcription followed by polymerase chain reaction (RT–PCR) is the most
suitable method for the detection and quantification of mRNA. It offers high sensitivity, good …

The stability of standard gene expression is an elementary prerequisite for internal
standardisation of target gene expression data and many so called housekeeping genes …

Quantification of mRNAs using real-time polymerase chain reaction (PCR) by monitoring the
product formation with the fluorescent dye SYBR Green I is being extensively used in …

To investigate whether the tumor suppressor gene PTEN affects the activity of the androgen
receptor (AR), we monitored the expression of the apoptotic gene HA-Bax (inserted in an …

A microvolume fluorometer integrated with a thermal cycler was used to acquire DNA
melting curves during polymerase chain reaction by fluorescence monitoring of the double …

Following its invention 25 years ago, PCR has been adapted for numerous molecular
biology applications. Gene expression analysis by reverse-transcription quantitative PCR …