Perspectives and benefits of high-throughput long-read sequencing in microbial ecology
Short-read, high-throughput sequencing (HTS) methods have yielded numerous important
insights into microbial ecology and function. Yet, in many instances short-read HTS …
insights into microbial ecology and function. Yet, in many instances short-read HTS …
Long-read sequencing–a powerful tool in viral transcriptome research
Long-read sequencing (LRS) has become increasingly popular due to its strengths in de
novo assembly and in resolving complex DNA regions as well as in determining full-length …
novo assembly and in resolving complex DNA regions as well as in determining full-length …
[HTML][HTML] Genomic characterization of emergent pseudorabies virus in China reveals marked sequence divergence: evidence for the existence of two major genotypes
C Ye, QZ Zhang, ZJ Tian, H Zheng, K Zhao, F Liu… - Virology, 2015 - Elsevier
Recently pseudorabies outbreaks have occurred in many vaccinated farms in China. To
identify genetic characteristics of pseudorabies virus (PRV) strains, we obtained the …
identify genetic characteristics of pseudorabies virus (PRV) strains, we obtained the …
Long-read sequencing uncovers a complex transcriptome topology in varicella zoster virus
Background Varicella zoster virus (VZV) is a human pathogenic alphaherpesvirus harboring
a relatively large DNA molecule. The VZV transcriptome has already been analyzed by …
a relatively large DNA molecule. The VZV transcriptome has already been analyzed by …
Full-length isoform sequencing reveals novel transcripts and substantial transcriptional overlaps in a herpesvirus
Whole transcriptome studies have become essential for understanding the complexity of
genetic regulation. However, the conventionally applied short-read sequencing platforms …
genetic regulation. However, the conventionally applied short-read sequencing platforms …
Long-read isoform sequencing reveals a hidden complexity of the transcriptional landscape of herpes simplex virus type 1
In this study, we used the amplified isoform sequencing technique from Pacific Biosciences
to characterize the poly (A)+ fraction of the lytic transcriptome of the herpes simplex virus …
to characterize the poly (A)+ fraction of the lytic transcriptome of the herpes simplex virus …
Analysis of the recombination and evolution of the new type mutant pseudorabies virus XJ5 in China
L Jiang, J Cheng, H Pan, F Yang, X Zhu, J Wu, H Pan… - BMC genomics, 2024 - Springer
Pseudorabies have caused enormous economic losses in China's pig industry and have
recurred on many large pig farms since late 2011. The disease is caused by highly …
recurred on many large pig farms since late 2011. The disease is caused by highly …
Identification of herpesvirus transcripts from genomic regions around the replication origins
Long-read sequencing (LRS) techniques enable the identification of full-length RNA
molecules in a single run eliminating the need for additional assembly steps. LRS research …
molecules in a single run eliminating the need for additional assembly steps. LRS research …
Multi-platform sequencing approach reveals a novel transcriptome profile in pseudorabies virus
Third-generation sequencing is an emerging technology that is capable of solving several
problems that earlier approaches were not able to, including the identification of transcripts …
problems that earlier approaches were not able to, including the identification of transcripts …
Highly efficient CRISPR/Cas9-mediated cloning and functional characterization of gastric cancer-derived Epstein-Barr virus strains
ABSTRACT The Epstein-Barr virus (EBV) is etiologically linked to approximately 10% of
gastric cancers, in which viral genomes are maintained as multicopy episomes. EBV …
gastric cancers, in which viral genomes are maintained as multicopy episomes. EBV …