CRISPR for crop improvement: an update review
The availability of genome sequences for several crops and advances in genome editing
approaches has opened up possibilities to breed for almost any given desirable trait …
approaches has opened up possibilities to breed for almost any given desirable trait …
Genetic mechanisms of abiotic stress tolerance that translate to crop yield stability
MV Mickelbart, PM Hasegawa… - Nature Reviews …, 2015 - nature.com
Crop yield reduction as a consequence of increasingly severe climatic events threatens
global food security. Genetic loci that ensure productivity in challenging environments exist …
global food security. Genetic loci that ensure productivity in challenging environments exist …
A multipurpose toolkit to enable advanced genome engineering in plants
T Čermák, SJ Curtin, J Gil-Humanes, R Čegan… - The Plant …, 2017 - academic.oup.com
We report a comprehensive toolkit that enables targeted, specific modification of monocot
and dicot genomes using a variety of genome engineering approaches. Our reagents …
and dicot genomes using a variety of genome engineering approaches. Our reagents …
Targeted mutagenesis, precise gene editing, and site-specific gene insertion in maize using Cas9 and guide RNA
S Svitashev, JK Young, C Schwartz, H Gao… - Plant …, 2015 - academic.oup.com
Targeted mutagenesis, editing of endogenous maize (Zea mays) genes, and site-specific
insertion of a trait gene using clustered regularly interspaced short palindromic repeats …
insertion of a trait gene using clustered regularly interspaced short palindromic repeats …
High-frequency, precise modification of the tomato genome
Background The use of homologous recombination to precisely modify plant genomes has
been challenging, due to the lack of efficient methods for delivering DNA repair templates to …
been challenging, due to the lack of efficient methods for delivering DNA repair templates to …
Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew
Sequence-specific nucleases have been applied to engineer targeted modifications in
polyploid genomes, but simultaneous modification of multiple homoeoalleles has not been …
polyploid genomes, but simultaneous modification of multiple homoeoalleles has not been …
A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation
LG Lowder, D Zhang, NJ Baltes, JW Paul III… - Plant …, 2015 - academic.oup.com
The relative ease, speed, and biological scope of clustered regularly interspaced short
palindromic repeats (CRISPR)/CRISPR-associated Protein9 (Cas9)-based reagents for …
palindromic repeats (CRISPR)/CRISPR-associated Protein9 (Cas9)-based reagents for …
Principles, applications, and biosafety of plant genome editing using CRISPR-Cas9
K El-Mounadi, ML Morales-Floriano… - Frontiers in plant …, 2020 - frontiersin.org
The terms genome engineering, genome editing, and gene editing, refer to modifications
(insertions, deletions, substitutions) in the genome of a living organism. The most widely …
(insertions, deletions, substitutions) in the genome of a living organism. The most widely …
Genome editing in rice and wheat using the CRISPR/Cas system
Targeted genome editing nucleases, such as zinc-finger nucleases (ZFNs) and transcription
activator-like effector nucleases (TALENs), are powerful tools for understanding gene …
activator-like effector nucleases (TALENs), are powerful tools for understanding gene …
Multiplex and homologous recombination–mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9
NATURE BIoTECHNoloGy volume 31 NumBeR 8 AuGuST 2013 689 was inserted to
minimize adverse effects on bacterial growth16 resulting from potential leaky expression …
minimize adverse effects on bacterial growth16 resulting from potential leaky expression …