Protein O-linked β-N-acetylglucosamine (O-GlcNAc) modification (O-GlcNAcylation) is a
unique monosaccharide modification discovered in the early 1980s. With the technological …

Today, many thousands of recombinant proteins, ranging from cytosolic enzymes to
membrane-bound proteins, have been successfully produced in baculovirus-infected insect …

Glycosylation defects are a hallmark of many nervous system diseases. However, the
molecular and metabolic basis for this pathology is not fully understood. In this study, we …

Glycosyltransferases are ubiquitous enzymes that catalyze the assembly of glycoconjugates
throughout all kingdoms of nature. A long-standing problem is the rational design of probes …

Hundreds of mammalian nuclear and cytoplasmic proteins are reversibly glycosylated by O-
linked β-N-acetylglucosamine (O-GlcNAc) to regulate their function, localization, and …

Metabolomics aims to quantify all metabolites within an organism, thereby providing
valuable insight into the metabolism of cells. To study intracellular metabolites, they are first …

In recent years, the number of recombinant proteins used for therapeutic applications has
increased dramatically. Many of these applications involve complex glycoproteins and …

Hyaluronan accumulation on cancer cells and their surrounding stroma predicts an
unfavourable disease outcome, suggesting that hyaluronan enhances tumor growth and …

Hyaluronan (HA) is a linear glycosaminoglycan (GAG) of extracellular matrix (ECM)
synthesized by three hyaluronan synthases (HASes) at the plasma membrane using uridine …

Glycans are one of the fundamental classes of macromolecules and are involved in a broad
range of biological phenomena. A large variety of glycan structures can be synthesized …