CRISPR technologies and the search for the PAM-free nuclease
D Collias, CL Beisel - Nature communications, 2021 - nature.com
The ever-expanding set of CRISPR technologies and their programmable RNA-guided
nucleases exhibit remarkable flexibility in DNA targeting. However, this flexibility comes with …
nucleases exhibit remarkable flexibility in DNA targeting. However, this flexibility comes with …
CRISPR-Cas9 DNA base-editing and prime-editing
Many genetic diseases and undesirable traits are due to base-pair alterations in genomic
DNA. Base-editing, the newest evolution of clustered regularly interspaced short palindromic …
DNA. Base-editing, the newest evolution of clustered regularly interspaced short palindromic …
Chromothripsis as an on-target consequence of CRISPR–Cas9 genome editing
Genome editing has therapeutic potential for treating genetic diseases and cancer.
However, the currently most practicable approaches rely on the generation of DNA double …
However, the currently most practicable approaches rely on the generation of DNA double …
The CRISPR tool kit for genome editing and beyond
M Adli - Nature communications, 2018 - nature.com
CRISPR is becoming an indispensable tool in biological research. Once known as the
bacterial immune system against invading viruses, the programmable capacity of the Cas9 …
bacterial immune system against invading viruses, the programmable capacity of the Cas9 …
Development of a gene-editing approach to restore vision loss in Leber congenital amaurosis type 10
ML Maeder, M Stefanidakis, CJ Wilson, R Baral… - Nature medicine, 2019 - nature.com
Leber congenital amaurosis type 10 is a severe retinal dystrophy caused by mutations in the
CEP290 gene,. We developed EDIT-101, a candidate genome-editing therapeutic, to …
CEP290 gene,. We developed EDIT-101, a candidate genome-editing therapeutic, to …
The emerging and uncultivated potential of CRISPR technology in plant science
The application of clustered regularly interspaced short palindromic repeats (CRISPR) for
genetic manipulation has revolutionized life science over the past few years. CRISPR was …
genetic manipulation has revolutionized life science over the past few years. CRISPR was …
[HTML][HTML] In vivo delivery of CRISPR-Cas9 therapeutics: Progress and challenges
Within less than a decade since its inception, CRISPR-Cas9-based genome editing has
been rapidly advanced to human clinical trials in multiple disease areas. Although it is highly …
been rapidly advanced to human clinical trials in multiple disease areas. Although it is highly …
CRISPR-based technologies for the manipulation of eukaryotic genomes
The CRISPR-Cas9 RNA-guided DNA endonuclease has contributed to an explosion of
advances in the life sciences that have grown from the ability to edit genomes within living …
advances in the life sciences that have grown from the ability to edit genomes within living …
Delivery strategies of the CRISPR-Cas9 gene-editing system for therapeutic applications
The CRISPR-Cas9 genome-editing system is a part of the adaptive immune system in
archaea and bacteria to defend against invasive nucleic acids from phages and plasmids …
archaea and bacteria to defend against invasive nucleic acids from phages and plasmids …
CRISPR/Cas9-based genome editing for disease modeling and therapy: challenges and opportunities for nonviral delivery
Genome editing offers promising solutions to genetic disorders by editing DNA sequences
or modulating gene expression. The clustered regularly interspaced short palindromic …
or modulating gene expression. The clustered regularly interspaced short palindromic …