Tuning a high performing multiplexed-CRISPRi Pseudomonas putida strain to further enhance indigoidine production
In this study, a 14-gene edited Pseudomonas putida KT2440 strain for heterologous
indigoidine production was examined using three distinct omic datasets. Transcriptomic data …
indigoidine production was examined using three distinct omic datasets. Transcriptomic data …
Engineering of succinyl-CoA metabolism in view of succinylation regulation to improve the erythromycin production
X Ke, X Jiang, M Huang, X Tian, J Chu - Applied Microbiology and …, 2022 - Springer
As a novel protein post-translational modification (PTM), lysine succinylation is widely
involved in metabolism regulation by altering the activity of catalytic enzymes. Inactivating …
involved in metabolism regulation by altering the activity of catalytic enzymes. Inactivating …
Precise fermentation coupling with simultaneous separation strategy enables highly efficient and economical sophorolipids production
F Xu, Y Chen, X Zou, J Chu, X Tian - Bioresource Technology, 2023 - Elsevier
Sophorolipids (SLs) represent highly promising biosurfactants. However, its widespread
production and application encounter obstacles due to the significant costs involved. Here …
production and application encounter obstacles due to the significant costs involved. Here …
Ammonium sulfate supplementation enhances erythromycin biosynthesis by augmenting intracellular metabolism and precursor supply in Saccharopolyspora …
Y Yuan, F Xu, X Ke, J Lu, M Huang, J Chu - Bioprocess and Biosystems …, 2023 - Springer
In this study, the cellular metabolic mechanisms regarding ammonium sulfate
supplementation on erythromycin production were investigated by employing targeted …
supplementation on erythromycin production were investigated by employing targeted …
Degradation performance and bioinformatic analysis of graphite electrode electrolysis cells for erythromycin fermentation residue
J Zhou, Z Wu, S Wang, H Wu, L Shi, S Tian… - Journal of Cleaner …, 2023 - Elsevier
To remove antibiotics and alleviate the resistance threats from the land application of
erythromycin fermentation residues, this study evaluated the degradation of erythromycin by …
erythromycin fermentation residues, this study evaluated the degradation of erythromycin by …
Reconstruction of the Genome-Scale Metabolic Model of Saccharopolyspora erythraea and Its Application in the Overproduction of Erythromycin
F Xu, J Lu, X Ke, M Shao, M Huang, J Chu - Metabolites, 2022 - mdpi.com
Saccharopolyspora erythraea is considered to be an effective host for erythromycin.
However, little is known about the regulation in terms of its metabolism. To develop an …
However, little is known about the regulation in terms of its metabolism. To develop an …
Untargeted metabolomics combined with metabolic flux analysis reveals the mechanism of sodium citrate for high S-adenosyl-methionine production by Pichia …
W Xu, F Xu, W Song, L Dong, J Qian, M Huang - Fermentation, 2022 - mdpi.com
S-adenosyl-methionine (SAM) is crucial for organisms to maintain some physiological
functions. However, the inconsistency between high L-methionine feeding rate and yield …
functions. However, the inconsistency between high L-methionine feeding rate and yield …
Engineering the methyltransferase through inactivation of the gen K and gen L leads to a significant increase of gentamicin C1a production in an industrial strain of …
F Xu, X Zhang, L Liu, X Ke, J Wu, Y Guo, X Tian… - Bioprocess and …, 2022 - Springer
In this study, a single-component high-yielding Micromonospora echinospora strain 49-92S-
KL01 was constructed by deleting methyltransferase-encoding genes gen K and gen L. In 5 …
KL01 was constructed by deleting methyltransferase-encoding genes gen K and gen L. In 5 …
Proteomic profiling of the endogenous peptides of MRSA and MSSA
H Tu, F Xu, Y Cheng, Q Pan, X Cai, S Wang, S Ge… - PeerJ, 2021 - peerj.com
Staphylococcus aureus is a Gram-positive bacterium that can cause diverse skin and soft
tissue infections. Methicillin-resistant Staphylococcus aureus (MRSA) can cause more …
tissue infections. Methicillin-resistant Staphylococcus aureus (MRSA) can cause more …
Enhancing erythromycin production in Saccharopolyspora erythraea through rational engineering and fermentation refinement: A Design‐Build‐Test‐Learn approach
M Shao, F Xu, X Ke, M Huang, J Chu - Biotechnology Journal, 2024 - Wiley Online Library
Industrial production of bioactive compounds from actinobacteria, such as erythromycin and
its derivatives, faces challenges in achieving optimal yields. To this end, the Design‐Build …
its derivatives, faces challenges in achieving optimal yields. To this end, the Design‐Build …