In the quest for a formidable weapon against the SARS-CoV-2 pandemic, mRNA
therapeutics have stolen the spotlight. mRNA vaccines are a prime example of the benefits …

Lipid encapsulated messenger RNA (LNP mRNA) has garnered a significant amount of
interest from the pharmaceutical industry and general public alike. This attention has been …

Messenger RNA (mRNA) is a new class of therapeutic compounds. The current advances in
mRNA technology require the development of efficient analytical methods. In this work, we …

CRISPR/Cas9 is a powerful genome editing approach in which a Cas9 enzyme and a single
guide RNA (sgRNA) form a ribonucleoprotein complex effectively targeting site-specific …

Health authorities have highlighted the need to determine oligonucleotide aggregates.
However, existing technologies have limitations that have prevented the reliable analysis of …

The choice of alternative materials over stainless steel hardware in the construction of liquid
chromatography systems has unveiled the degree to which nonspecific adsorption impacts …

As interest in oligonucleotide (ON) therapeutics is increasing, there is a need to develop
suitable analytical methods able to properly analyze those molecules. However, an issue …

Certain biomolecules have proven to be difficult to analyze by liquid chromatography (LC),
especially under certain chromatographic conditions. The separation of proteins in aqueous …

The adsorptive loss of acidic analytes in liquid chromatography was investigated using
metal frits. Repetitive injections of acidic small molecules or an oligonucleotide were made …

In this study we investigated the separation of a 25 mer fully phosphorothioated
oligonucleotide from its truncated n-1 (24 mer) species and selected phosphodiester 25 mer …