Controlling and enhancing CRISPR systems
Many bacterial and archaeal organisms use clustered regularly interspaced short
palindromic repeats–CRISPR associated (CRISPR–Cas) systems to defend themselves …
palindromic repeats–CRISPR associated (CRISPR–Cas) systems to defend themselves …
CRISPR-Cas systems: prokaryotes upgrade to adaptive immunity
R Barrangou, LA Marraffini - Molecular cell, 2014 - cell.com
Clustered regularly interspaced short palindromic repeats (CRISPR), and associated
proteins (Cas) comprise the CRISPR-Cas system, which confers adaptive immunity against …
proteins (Cas) comprise the CRISPR-Cas system, which confers adaptive immunity against …
RNA-guided genetic silencing systems in bacteria and archaea
Clustered regularly interspaced short palindromic repeat (CRISPR) are essential
components of nucleic-acid-based adaptive immune systems that are widespread in …
components of nucleic-acid-based adaptive immune systems that are widespread in …
CRISPR-mediated adaptive immune systems in bacteria and archaea
R Sorek, CM Lawrence… - Annual review of …, 2013 - annualreviews.org
Effective clearance of an infection requires that the immune system rapidly detects and
neutralizes invading parasites while strictly avoiding self-antigens that would result in …
neutralizes invading parasites while strictly avoiding self-antigens that would result in …
CRISPR-Cas systems in bacteria and archaea: versatile small RNAs for adaptive defense and regulation
D Bhaya, M Davison, R Barrangou - Annual review of genetics, 2011 - annualreviews.org
Bacteria and archaea have evolved defense and regulatory mechanisms to cope with
various environmental stressors, including virus attack. This arsenal has been expanded by …
various environmental stressors, including virus attack. This arsenal has been expanded by …
Insertion sequence transposition inactivates CRISPR-Cas immunity
Y Sheng, H Wang, Y Ou, Y Wu, W Ding, M Tao… - Nature …, 2023 - nature.com
CRISPR-Cas immunity systems safeguard prokaryotic genomes by inhibiting the invasion of
mobile genetic elements. Here, we screened prokaryotic genomic sequences and identified …
mobile genetic elements. Here, we screened prokaryotic genomic sequences and identified …
CRISPR immunity relies on the consecutive binding and degradation of negatively supercoiled invader DNA by Cascade and Cas3
The prokaryotic CRISPR/Cas immune system is based on genomic loci that contain
incorporated sequence tags from viruses and plasmids. Using small guide RNA molecules …
incorporated sequence tags from viruses and plasmids. Using small guide RNA molecules …
Biogenesis pathways of RNA guides in archaeal and bacterial CRISPR-Cas adaptive immunity
E Charpentier, H Richter, J van der Oost… - FEMS microbiology …, 2015 - academic.oup.com
CRISPR-Cas is an RNA-mediated adaptive immune system that defends bacteria and
archaea against mobile genetic elements. Short mature CRISPR RNAs (crRNAs) are key …
archaea against mobile genetic elements. Short mature CRISPR RNAs (crRNAs) are key …
Molecular memory of prior infections activates the CRISPR/Cas adaptive bacterial immunity system
KA Datsenko, K Pougach, A Tikhonov… - Nature …, 2012 - nature.com
Abstract CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-
associated genes) is a small RNA-based adaptive prokaryotic immunity system that …
associated genes) is a small RNA-based adaptive prokaryotic immunity system that …
Protospacer recognition motifs: mixed identities and functional diversity
Protospacer adjacent motifs (PAMs) were originally characterized for CRISPR-Cas systems
that were classified on the basis of their CRISPR repeat sequences. A few short 2–5 bp …
that were classified on the basis of their CRISPR repeat sequences. A few short 2–5 bp …