Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …

Eukaryotic cells deploy overlapping repair pathways to resolve DNA damage.
Advancements in genome editing take advantage of these pathways to produce permanent …

The DNA mutation produced by cellular repair of a CRISPR–Cas9-generated double-strand
break determines its phenotypic effect. It is known that the mutational outcomes are not …

CRISPR-Cas genome editing induces targeted DNA damage but can also affect off-target
sites. Current off-target discovery methods work using purified DNA or specific cellular …

The CRISPR-Cas9 system has successfully been adapted to edit the genome of various
organisms. However, our ability to predict the editing outcome at specific sites is limited …

Investigations of CRISPR gene knockout editing profiles have contributed to enhanced
precision of editing outcomes. However, for homology-directed repair (HDR) in particular …

Background Germline mutations in the BRCA1 or BRCA2 (BRCA) genes predispose to
hereditary breast and ovarian cancer and, mostly in the case of BRCA2, are also prevalent …

Genome editing, specifically CRISPR/Cas9 technology, has revolutionized biomedical
research and offers potential cures for genetic diseases. Despite rapid progress, low …

Advances in genome editing technologies have enabled manipulation of genomes at the
single base level. These technologies are based on programmable nucleases (PNs) that …

During CRISPR-directed gene editing, multiple gene repair mechanisms interact to produce
a wide and largely unpredictable variety of sequence changes across an edited population …