During development and throughout life, a variety of specialized cells must be generated to
ensure the proper function of each tissue and organ. Chromatin plays a key role in …

Currently, two main cell culture models predominate pluripotent stem cell research:
embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). Thanks to their …

Totipotent cells have more robust developmental potency than any other cell types, giving
rise to both embryonic and extraembryonic tissues. Stable totipotent cell cultures and …

The activation of the embryonic genome marks the first major wave of transcription in the
developing organism. Zygotic genome activation (ZGA) in mouse 2-cell embryos and 8-cell …

Since establishment of the first embryonic stem cells (ESCs), in vitro culture of totipotent cells
functionally and molecularly comparable with in vivo blastomeres with embryonic and …

Totipotency is the ability of a single cell to give rise to all of the differentiated cell types that
build the conceptus, yet how to capture this property in vitro remains incompletely …

It is challenging to derive totipotent stem cells in vitro that functionally and molecularly
resemble cells from totipotent embryos. Here, we report that a chemical cocktail enables the …

Abstract N6-methyladenosine (m6A) on chromosome-associated regulatory RNAs
(carRNAs), including repeat RNAs, plays important roles in tuning the chromatin state and …

In mammalian embryos, proper zygotic genome activation (ZGA) underlies totipotent
development. Double homeobox (DUX)-family factors participate in ZGA, and mouse Dux is …

Totipotency emerges in early embryogenesis, but its molecular underpinnings remain poorly
characterized. In the present study, we employed DNA fiber analysis to investigate how …