Bioorthogonal chemistry represents a class of high-yielding chemical reactions that proceed
rapidly and selectively in biological environments without side reactions towards …

The emerging inverse electron demand Diels–Alder (IEDDA) reaction stands out from other
bioorthogonal reactions by virtue of its unmatchable kinetics, excellent orthogonality and …

The selective chemical modification of biological molecules drives a good portion of modern
drug development and fundamental biological research. While a few early examples of …

The ideal fluorescent probe for bioimaging is bright, absorbs at long wavelengths and can
be implemented flexibly in living cells and in vivo. However, the design of synthetic …

The tetrazine/trans-cyclooctene ligation stands out from the bioorthogonal toolbox due to its
exceptional reaction kinetics, enabling multiple molecular technologies in vitro and in living …

Inverse electron demand Diels–Alder reactions (iEDDA) between 1, 2, 4, 5-tetrazines and
olefins have emerged into a state-of-the art concept for the conjugation of biomolecules …

One of the most promising strategies for the controlled release of therapeutic molecules is
stimuli-responsive and biodegradable hydrogels developed from natural polymers …

Bioorthogonal chemistries can be used to tag diverse classes of biomolecules in cells and
other complex environments. With over 20 unique transformations now available, though …

Conspectus Bioorthogonal chemistry has found increased application in living systems over
the past decade. In particular, tetrazine bioorthogonal chemistry has become a powerful tool …

The fastest and the brightest: A new design that intimately connects tetrazine to a BODIPY
fluorophore enables exceptionally efficient energy transfer and quenching. Upon reaction of …