Plasmid evolution in carbapenemase‐producing Enterobacteriaceae: a review

K Kopotsa, J Osei Sekyere… - Annals of the New York …, 2019 - Wiley Online Library
Carbapenem‐resistant Enterobacteriaceae (CRE) have been listed by the WHO as high‐
priority pathogens owing to their high association with mortalities and morbidities …

Optical DNA mapping in nanofluidic devices: principles and applications

V Müller, F Westerlund - Lab on a Chip, 2017 - pubs.rsc.org
Optical DNA mapping has over the last decade emerged as a very powerful tool for
obtaining long range sequence information from single DNA molecules. In optical DNA …

Plasmid classification in an era of whole-genome sequencing: application in studies of antibiotic resistance epidemiology

A Orlek, N Stoesser, MF Anjum, M Doumith… - Frontiers in …, 2017 - frontiersin.org
Plasmids are extra-chromosomal genetic elements ubiquitous in bacteria, and commonly
transmissible between host cells. Their genomes include variable repertoires of 'accessory …

DNA in nanochannels: theory and applications

K Frykholm, V Müller, KK Sriram… - Quarterly Reviews of …, 2022 - cambridge.org
Nanofluidic structures have over the last two decades emerged as a powerful platform for
detailed analysis of DNA on the kilobase pair length scale. When DNA is confined to a …

Single-molecule optical genome mapping in nanochannels: multidisciplinarity at the nanoscale

J Jeffet, S Margalit, Y Michaeli… - Essays in …, 2021 - portlandpress.com
The human genome contains multiple layers of information that extend beyond the genetic
sequence. In fact, identical genetics do not necessarily yield identical phenotypes as evident …

Direct identification of antibiotic resistance genes on single plasmid molecules using CRISPR/Cas9 in combination with optical DNA mapping

V Müller, F Rajer, K Frykholm, LK Nyberg, S Quaderi… - Scientific reports, 2016 - nature.com
Bacterial plasmids are extensively involved in the rapid global spread of antibiotic
resistance. We here present an assay, based on optical DNA mapping of single plasmids in …

[HTML][HTML] PlasmidSeeker: identification of known plasmids from bacterial whole genome sequencing reads

M Roosaare, M Puustusmaa, M Möls, M Vaher… - PeerJ, 2018 - peerj.com
Background Plasmids play an important role in the dissemination of antibiotic resistance,
making their detection an important task. Using whole genome sequencing (WGS), it is …

Phosphorylated CtIP bridges DNA to promote annealing of broken ends

R Öz, SM Howard, R Sharma… - Proceedings of the …, 2020 - National Acad Sciences
The early steps of DNA double-strand break (DSB) repair in human cells involve the MRE11-
RAD50-NBS1 (MRN) complex and its cofactor, phosphorylated CtIP. The roles of these …

Strain-level bacterial typing directly from patient samples using optical DNA mapping

M Nyblom, A Johnning, K Frykholm, M Wrande… - Communications …, 2023 - nature.com
Background Identification of pathogens is crucial to efficiently treat and prevent bacterial
infections. However, existing diagnostic techniques are slow or have a too low resolution for …

Optical DNA mapping combined with Cas9-targeted resistance gene identification for rapid tracking of resistance plasmids in a neonatal intensive care unit outbreak

SK Bikkarolla, V Nordberg, F Rajer, V Müller, MH Kabir… - MBio, 2019 - Am Soc Microbiol
The global spread of antibiotic resistance among Enterobacteriaceae is largely due to
multidrug resistance plasmids that can transfer between different bacterial strains and …