The emergence of bioorthogonal reactions has greatly broadened the scope of biomolecule
labeling and detecting. Of all the bioorthogonal reactions that have been developed, the …

The copper-catalyzed azide–alkyne cycloaddition (CuAAC) is one of the most broadly
applicable and easy-to-handle reactions in the arsenal of organic chemistry. However, the …

Bioorthogonal phosphines were introduced in the context of the Staudinger ligation over 20
years ago. Since that time, phosphine probes have been used in myriad applications to tag …

Base‐modified fluorescent nucleoside analog probes have been very valuable in the study
of nucleic acid structure and function. Many of them structurally resemble natural bases, and …

Abstract The use of 'Click Reaction'for Cu (I) catalyzed azide–alkyne cycloaddition (CuAAC)
has emerged as one of the most powerful tools for the synthesis of library of organic …

One of the major constraints on the therapeutic use of oligonucleotides is inefficient delivery
to their sites of action in the cytosol or nucleus. Recently it has become evident that the …

Cellular RNA labeling strategies based on bioorthogonal chemical reactions are much less
developed in comparison to glycan, protein and DNA due to its inherent instability and lack …

To understand the structure and ensuing function of RNA in various cellular processes,
researchers greatly rely on traditional as well as contemporary labeling technologies to …

5-Azidomethyl dUTP and two 5-trans-cyclooctene dUTPs with different linkers between the
TCO and the uracil base have been incorporated into DNA by primer extension, reverse …

Here, we present a simple, modular and efficient strategy that allows the 3′-terminal
labeling of DNA, regardless of whether it has been chemically or enzymatically synthesized …