Plant genome sequence assembly in the era of long reads: Progress, challenges and future directions

B Pucker, I Irisarri, J de Vries, B Xu - Quantitative Plant Biology, 2022 - cambridge.org
Third-generation long-read sequencing is transforming plant genomics. Oxford Nanopore
Technologies and Pacific Biosciences are offering competing long-read sequencing …

Telomere-to-telomere assemblies of 142 strains characterize the genome structural landscape in Saccharomyces cerevisiae

S O'donnell, JX Yue, OA Saada, N Agier, C Caradec… - Nature Genetics, 2023 - nature.com
Pangenomes provide access to an accurate representation of the genetic diversity of
species, both in terms of sequence polymorphisms and structural variants (SVs). Here we …

The Chlamydomonas Genome Project, version 6: Reference assemblies for mating-type plus and minus strains reveal extensive structural mutation in the laboratory

RJ Craig, SD Gallaher, S Shu, PA Salomé… - The Plant …, 2023 - academic.oup.com
Five versions of the Chlamydomonas reinhardtii reference genome have been produced
over the last two decades. Here we present version 6, bringing significant advances in …

A gap-free genome assembly of Chlamydomonas reinhardtii and detection of translocations induced by CRISPR-mediated mutagenesis

ZL Payne, GM Penny, TN Turner, SK Dutcher - Plant Communications, 2023 - cell.com
Genomic assemblies of the unicellular green alga Chlamydomonas reinhardtii have
provided important resources for researchers. However, assembly errors, large gaps, and …

Chloroplast ATP synthase biogenesis requires peripheral stalk subunits AtpF and ATPG and stabilization of atpE mRNA by OPR protein MDE1

F Chaux, D Jarrige, M Rodrigues‐Azevedo… - The Plant …, 2023 - Wiley Online Library
Chloroplast ATP synthase contains subunits of plastid and nuclear genetic origin. To
investigate the coordinated biogenesis of this complex, we isolated novel ATP synthase …

An Ancient Clade of Penelope-Like Retroelements with Permuted Domains Is Present in the Green Lineage and Protists, and Dominates Many Invertebrate Genomes

RJ Craig, IA Yushenova, F Rodriguez… - … Biology and Evolution, 2021 - academic.oup.com
Penelope-like elements (PLEs) are an enigmatic clade of retrotransposons whose reverse
transcriptases (RTs) share a most recent common ancestor with telomerase RTs. The single …

The distribution of fitness effects of spontaneous mutations in Chlamydomonas reinhardtii inferred using frequency changes under experimental evolution

KB Böndel, T Samuels, RJ Craig, RW Ness… - PLoS …, 2022 - journals.plos.org
The distribution of fitness effects (DFE) for new mutations is fundamental for many aspects of
population and quantitative genetics. In this study, we have inferred the DFE in the single …

Telomerase-independent survival leads to a mosaic of complex subtelomere rearrangements in Chlamydomonas reinhardtii

F Chaux, N Agier, C Garrido, G Fischer… - Genome …, 2023 - genome.cshlp.org
Telomeres and subtelomeres, the genomic regions located at chromosome extremities, are
essential for genome stability in eukaryotes. In the absence of the canonical maintenance …

Diverse populations of Staphylococcus pseudintermedius colonize the skin of healthy dogs

N Fàbregas, D Pérez, J Viñes, A Cuscó… - Microbiology …, 2023 - Am Soc Microbiol
Staphylococcus pseudintermedius is a commensal bacterium of the canine skin but is also a
key opportunistic pathogen that is responsible for most cases of pyoderma in dogs. The …

Phylogenetic profiling resolves early emergence of PRC2 and illuminates its functional core

A Sharaf, M Vijayanathan, M Oborník… - Life Science …, 2022 - life-science-alliance.org
Polycomb repressive complex 2 (PRC2) is involved in maintaining transcriptionally silent
chromatin states through methylating lysine 27 of histone H3 by the catalytic subunit …