HIV-1 is dependent on its immature lattice to recruit IP6 for mature capsid assembly
N Renner, A Kleinpeter, DL Mallery… - Nature structural & …, 2023 - nature.com
HIV-1 Gag metamorphoses inside each virion, from an immature lattice that forms during
viral production to a mature capsid that drives infection. Here we show that the immature …
viral production to a mature capsid that drives infection. Here we show that the immature …
Biochemical reconstitution of HIV-1 assembly and maturation
The assembly of an orthoretrovirus such as HIV-1 requires the coordinated functioning of
multiple biochemical activities of the viral Gag protein. These activities include membrane …
multiple biochemical activities of the viral Gag protein. These activities include membrane …
Computational Enzyme Redesign Enhances Tolerance to Denaturants for Peptide C-Terminal Amidation
The escalating demand for biocatalysts in pharmaceutical and biochemical applications
underscores the critical imperative to enhance enzyme activity and durability under high …
underscores the critical imperative to enhance enzyme activity and durability under high …
Recombinant expression of HIV-1 protease using soluble fusion tags in Escherichia coli: A vital tool for functional characterization of HIV-1 protease
HIV-1 protease expression in the laboratory is demanding because of its high cytotoxicity,
making it difficult to express in bacterial expression systems such as Escherichia coli. To …
making it difficult to express in bacterial expression systems such as Escherichia coli. To …
Purification and characterization of naturally occurring HIV-1 (South African subtype C) protease mutants from inclusion bodies
Human immunodeficiency virus (HIV) infections in sub-Saharan Africa represent about 56%
of global infections. Many studies have targeted HIV-1 protease for the development of …
of global infections. Many studies have targeted HIV-1 protease for the development of …
Improved expression and purification of highly-active 3 chymotrypsin-like protease from SARS-CoV-2
HLT Nguyen, NQT Nguyen, TT Le, XDT Pham… - Protein Expression and …, 2024 - Elsevier
Severe acute respiratory syndrome–coronavirus 2 (SARS-CoV-2) is the causative pathogen
of coronavirus disease-19 (COVID-19). The COVID-19 pandemic has resulted in millions of …
of coronavirus disease-19 (COVID-19). The COVID-19 pandemic has resulted in millions of …
mRNA Engineering for the Efficient Chaperone-Mediated Co-Translational Folding of Recombinant Proteins in Escherichia coli
The production of soluble, functional recombinant proteins by engineered bacterial hosts is
challenging. Natural molecular chaperone systems have been used to solubilize various …
challenging. Natural molecular chaperone systems have been used to solubilize various …
Optimized procedure for recovering HIV-1 protease (C-SA) from inclusion bodies
HIV-1 is an infectious virus that causes acquired immunodeficiency syndrome (AIDS) and it
is one of the major causes of deaths worldwide. The production of HIV-1 protease (PR) on a …
is one of the major causes of deaths worldwide. The production of HIV-1 protease (PR) on a …
Refolding and purification of cGMP-grade recombinant human neurturin from Escherichia coli inclusion bodies
G Xi, R Esfandiary, CB Sacramento, H Jouihan… - Protein Expression and …, 2020 - Elsevier
Neurturin is a potent neurotrophic factor that has been investigated as a potential
therapeutic agent for the treatment of neurodegenerative diseases, including Parkinson's …
therapeutic agent for the treatment of neurodegenerative diseases, including Parkinson's …
Identification of a novel HIV-1C protease from a microbial source
Z Sonday - 2023 - open.uct.ac.za
HIV-1 subtype C is currently the most prevalent in the epidemiology of HIV/AIDS cases in
subSaharan Africa. Most clinical protease inhibitors (PIs) were designed against subtype B …
subSaharan Africa. Most clinical protease inhibitors (PIs) were designed against subtype B …