Expansion microscopy: principles and uses in biological research

AT Wassie, Y Zhao, ES Boyden - Nature methods, 2019 - nature.com
Many biological investigations require 3D imaging of cells or tissues with nanoscale spatial
resolution. We recently discovered that preserved biological specimens can be physically …

Current progress in expansion microscopy: chemical strategies and applications

G Wen, V Leen, T Rohand, M Sauer… - Chemical …, 2023 - ACS Publications
Expansion microscopy (ExM) is a newly developed super-resolution technique, allowing
visualization of biological targets at nanoscale resolution on conventional fluorescence …

Wood-derived hydrogels as a platform for drug-release systems

M Culebras, A Barrett, M Pishnamazi… - ACS sustainable …, 2021 - ACS Publications
Wood (cellulose and lignin)-based hydrogels were successfully produced as platforms for
drug-release systems. Viscoelastic and cross-linking behaviors of precursor solutions were …

Tuning the range of polyacrylamide gel stiffness for mechanobiology applications

AK Denisin, BL Pruitt - ACS applied materials & interfaces, 2016 - ACS Publications
Adjusting the acrylamide monomer and cross-linker content in polyacrylamide gels controls
the hydrogel stiffness, yet the reported elastic modulus for the same formulations varies …

Expansion microscopy: protocols for imaging proteins and RNA in cells and tissues

SM Asano, R Gao, AT Wassie… - Current protocols in …, 2018 - Wiley Online Library
Expansion microscopy (ExM) is a recently developed technique that enables nanoscale‐
resolution imaging of preserved cells and tissues on conventional diffraction‐limited …

Cells lying on a bed of microneedles: an approach to isolate mechanical force

JL Tan, J Tien, DM Pirone, DS Gray… - Proceedings of the …, 2003 - National Acad Sciences
We describe an approach to manipulate and measure mechanical interactions between
cells and their underlying substrates by using microfabricated arrays of elastomeric …

Physical chemistry of supramolecular polymer networks

S Seiffert, J Sprakel - Chemical Society Reviews, 2012 - pubs.rsc.org
Supramolecular polymer networks are three-dimensional structures of crosslinked
macromolecules connected by transient, non-covalent bonds; they are a fascinating class of …

Spatial inhomogeneity and dynamic fluctuations of polymer gels

M Shibayama - Macromolecular Chemistry and Physics, 1998 - Wiley Online Library
Polymer gels have two types of concentration fluctuations; liquidlike and solidlike
fluctuations. The former are time‐dependent (thermal) fluctuations and the latter are time …

Progress toward robust polymer hydrogels

S Naficy, HR Brown, JM Razal… - Australian Journal of …, 2011 - CSIRO Publishing
In this review we highlight new developments in tough hydrogel materials in terms of their
enhanced mechanical performance and their corresponding toughening mechanisms …

A highly homogeneous polymer composed of tetrahedron-like monomers for high-isotropy expansion microscopy

R Gao, CC Yu, L Gao, KD Piatkevich, RL Neve… - Nature …, 2021 - nature.com
Expansion microscopy (ExM) physically magnifies biological specimens to enable
nanoscale-resolution imaging using conventional microscopes. Current ExM methods …