Serial block face scanning electron microscopy in cell biology: Applications and technology
D Smith, T Starborg - Tissue and Cell, 2019 - Elsevier
Three-dimensional electron microscopy (3DEM) is an imaging field containing several
powerful modalities such as serial section transmission electron microscopy and electron …
powerful modalities such as serial section transmission electron microscopy and electron …
Embracing 3D complexity in leaf carbon–water exchange
Leaves are a nexus for the exchange of water, carbon, and energy between terrestrial plants
and the atmosphere. Research in recent decades has highlighted the critical importance of …
and the atmosphere. Research in recent decades has highlighted the critical importance of …
Electron microscopy using the genetically encoded APEX2 tag in cultured mammalian cells
JD Martell, TJ Deerinck, SS Lam, MH Ellisman… - Nature protocols, 2017 - nature.com
Electron microscopy (EM) is the premiere technique for high-resolution imaging of cellular
ultrastructure. Unambiguous identification of specific proteins or cellular compartments in …
ultrastructure. Unambiguous identification of specific proteins or cellular compartments in …
Morphological bases of phytoplankton energy management and physiological responses unveiled by 3D subcellular imaging
Eukaryotic phytoplankton have a small global biomass but play major roles in primary
production and climate. Despite improved understanding of phytoplankton diversity and …
production and climate. Despite improved understanding of phytoplankton diversity and …
X-ray microscopy enables multiscale high-resolution 3D imaging of plant cells, tissues, and organs
Capturing complete internal anatomies of plant organs and tissues within their relevant
morphological context remains a key challenge in plant science. While plant growth and …
morphological context remains a key challenge in plant science. While plant growth and …
The bias of a two‐dimensional view: comparing two‐dimensional and three‐dimensional mesophyll surface area estimates using noninvasive imaging
The mesophyll surface area exposed to intercellular air space per leaf area (Sm) is closely
associated with CO2 diffusion and photosynthetic rates. Sm is typically estimated from two …
associated with CO2 diffusion and photosynthetic rates. Sm is typically estimated from two …
Advanced biofilm staining techniques for TEM and SEM in geomicrobiology: Implications for visualizing EPS architecture, mineral nucleation, and microfossil …
J McCutcheon, G Southam - Chemical Geology, 2018 - Elsevier
Microbial biofilms and mats have long been studied for their role in mineral precipitation
reactions in natural environments. Scanning electron microscopy (SEM) is often used to …
reactions in natural environments. Scanning electron microscopy (SEM) is often used to …
Cell and chloroplast anatomical features are poorly estimated from 2D cross‐sections
R Harwood, E Goodman, M Gudmundsdottir… - New …, 2020 - Wiley Online Library
Leaf function is intimately related to the size, shape, abundance and position of cells and
chloroplasts. Anatomy has long been assessed and quantified in two dimensions with 3D …
chloroplasts. Anatomy has long been assessed and quantified in two dimensions with 3D …
Protein Storage Vacuoles Originate from Remodeled Preexisting Vacuoles in Arabidopsis thaliana
Protein storage vacuoles (PSV) are the main repository of protein in dicotyledonous seeds,
but little is known about the origins of these transient organelles. PSV are hypothesized to …
but little is known about the origins of these transient organelles. PSV are hypothesized to …
Serial block-face scanning electron microscopy (SBF-SEM) of biological tissue samples
JA Courson, PT Landry, T Do, E Spehlmann… - JoVE (Journal of …, 2021 - jove.com
Serial Block-Face Scanning Electron Microscopy (SBF-SEM) of Biological Tissue Samples -
JoVE JoVE JoVE Faculty Resource Center Research Behavior Biochemistry Biology …
JoVE JoVE JoVE Faculty Resource Center Research Behavior Biochemistry Biology …