Escherichia coli is the most widely used heterologous protein expression system. However,
this system remains a challenge due to the low solubility of proteins, insufficient yield, and …

Escherichia coli has been the most widely used host for the production of recombinant
proteins because it is the best characterized system in every aspect. Furthermore, the high …

Human interferon gamma is a cytokine belonging to a diverse group of interferons which
have a crucial immunological function against mycobacteria and a wide variety of viral …

Abstract Development of successful production strategies for recombinant proteins is a
challenging task due to problems associated with the scale‐up of bioprocesses. Recent …

Different hosts have been used for recombinant protein production, ranging from simple
bacteria, such as Escherichia coli and Bacillus subtilis, to more advanced eukaryotes as …

The demand for therapeutic recombinant proteins is set to see a significant increase over the
next few years. As a consequence, the processes used to produce these proteins must be …

This paper reviews recent strategies used for increasing specific yield and productivity in
high cell density cultures. High cell density cultures offer an efficient means for the …

Abstract Background Inclusion bodies (IBs) are aggregated proteins that form clusters when
protein is overexpressed in heterologous expression systems. IBs have been considered as …

Sažetak Microbial fermentation processes are of great importance for the production of many
bioproducts. Even for established processes, improvements in product yield, productivity …

The Red recombinase system, the most convenient genetic tool applied in Escherichia coli
and other bacteria, was introduced for gene replacement in Klebsiella pneumoniae. The …